ArticleJournal of cellular and molecular medicine2020
Optimized Langendorff perfusion system for cardiomyocyte isolation in adult mouse heart.
Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Presentation of preload and afterload to isolated heart preparations.The Journal of physiology · 2026Review
- A Simplified Langendorff-Based Method for Mouse Cardiac Myocyte Isolation.Bio-protocol · 2026Article
- Membrane Oxygenation Improves Functional Myocardial Preservation and Enables Colloid-Enriched Perfusion in the Langendorff Isolated Heart Model.Medical sciences (Basel, Switzerland) · 2026Article
- Cardiac epigenome in heart development and disease.Nature reviews. Cardiology · 2026Review
- An Integrated Evaluation Framework for Adult Heart Regeneration.Journal of cellular and molecular medicine · 2026Article
- Single-cell transcriptomic profiling reveals cell type heterogeneity between HFpEF and HFrEF.Communications biology · 2025Article
- Distinct morphometric features of cardiomyocytes isolated from mouse hypertrophy models: An ImageJ analysis combined with machine learning algorithms.Physiological reports · 2025Article
- Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in mice.Nature communications · 2025Article
- Mitochondrial metabolic maturation in postnatal right ventricle restricted by volume overload.Intractable & rare diseases research · 2025Article
- An optimized Langendorff-free method for isolation and characterization of primary adult cardiomyocytes.BMC cardiovascular disorders · 2024Article
- Activation of the nonneuronal cholinergic cardiac system by hypoxic preconditioning protects isolated adult cardiomyocytes from hypoxia/reoxygenation injury.American journal of physiology. Heart and circulatory physiology · 2024Article
- An Optimized Langendorff-free Method for Isolation and Characterization of Primary Adult Cardiomyocytes.Research square · 2024Article
- Electrophysiological evaluation of the effect of peptide toxins on voltage-gated ion channels: a scoping review on theoretical and methodological aspects with focus on the Central and South American experience.The journal of venomous animals and toxins including tropical diseases · 2024Article
- Comparative Analysis of Heart Regeneration: Searching for the Key to Heal the Heart-Part I: Experimental Injury Models to Study Cardiac Regeneration.Journal of cardiovascular development and disease · 2023Review
- Omentin-1 drives cardiomyocyte cell cycle arrest and metabolic maturation by interacting with BMP7.Cellular and molecular life sciences : CMLS · 2023Article
- Nogo-A reduces ceramide de novo biosynthesis to protect from heart failure.Cardiovascular research · 2023Article
- Myocardial infarction from a tissue engineering and regenerative medicine point of view: A comprehensive review on models and treatments.Biophysics reviews · 2022Review
- Protocol to record and quantify the intracellular pH in contracting cardiomyocytes.STAR protocols · 2022Article
- Molecular Changes in Prepubertal Left Ventricular Development Under Experimental Volume Overload.Frontiers in cardiovascular medicine · 2022Article
- Postnatal Right Ventricular Developmental Track Changed by Volume Overload.Journal of the American Heart Association · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the rapid development of single-cell sequencing technology, the Langendorff perfusion system has emerged as a common approach to decompose cardiac tissue and obtain living cardiomyocytes to study cardiovascular disease with the mechanism of cardiomyocyte biology. However, the traditional Langendorff perfusion system is difficult to master, and further, the viability and purity of cardiomyocytes are frequently unable to meet sequencing requirements due to complicated devices and manipulate processes. Here, we provide an optimized Langendorff perfusion system with a simplified and standardized operating protocol which utilizes gravity as the perfusion pressure, includes a novel method for bubbles removing and standardizes the criteria for termination of digestion. We obtained stable cardiomyocyte with high viability and purity after multiple natural gravity sedimentation. The combination of the optimized Langendorff perfusion system and the multiple natural gravity sedimentation provides a stable system for isolating adult mouse heart, which will provide higher-quality cardiomyocytes for further experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.