Evidence map›Paper›PMID 33146981›Full record

ArticleInternational braz j urol : official journal of the Brazilian Society of Urology

Oxidative origin of sperm DNA fragmentation in the adult varicocele.

Jessica Timóteo Jeremias, Larissa Berloffa Belardin, Fatima Kazue Okada, Mariana P Antoniassi, Renato Fraietta, Ricardo Pimenta Bertolla, Paula Intasqui

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International braz j urol : official journal of the Brazilian Society of Urology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
5.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jessica Timóteo JeremiasDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Larissa Berloffa BelardinDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Fatima Kazue OkadaDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Mariana P AntoniassiDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Renato FraiettaDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Ricardo Pimenta BertollaDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Paula IntasquiDepartamento de Cirurgia, Divisão de Urologia, Seção de Reprodução Humana, Universidade Federal de São Paulo - Unifesp, São Paulo, SP, Brasil.
Fundação de Apoio à Universidade Federal de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeSperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. MATERIALS AND

methodsA cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student's t test or a Mann-Whitney test.

resultsThe varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidative-induced DNA fragmentation.

conclusionsPatients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage.

Indexed as

Infertility, MaleVaricoceleAdultCross-Sectional StudiesDNA FragmentationHumansMaleOxidative StressSpermatozoaSperm MotilityDNA FragmentationInfertility, MaleOxidative Stress

Identifiers

PMID33146981
PMCPMC7857753
OpenAlexW3096418656

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.