Evidence map›Paper›PMID 33146708›Full record

ArticleBioscience reports2020

Studies on the changes of uPA system in a co-culture model of bone marrow stromal cells-leukemia cells.

Lanxia Zhou, Hong Guo, Fang Jia, Xuan Chen, Xiaowei Zhang, Shouliang Dong, Li Zhao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Lanxia Zhou *The Central Laboratory, The First Hospital, Lanzhou University, Lanzhou, Gansu, China.
Hong Guo *Critical Care Medicine Department, The First Hospital, Lanzhou University, Lanzhou, Gansu, China.
Fang Jia *Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, China.
Xuan ChenThe Central Laboratory, The First Hospital, Lanzhou University, Lanzhou, Gansu, China.
Xiaowei ZhangThe Central Laboratory, The First Hospital, Lanzhou University, Lanzhou, Gansu, China.
Shouliang DongInstitute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, China.
Li ZhaoThe Central Laboratory, The First Hospital, Lanzhou University, Lanzhou, Gansu, China.
First Hospital of Lanzhou University · CNLanzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The core of the tumor microenvironment in the hematological system is formed by bone marrow stromal cells (BMSCs). In the present study, we explored the interaction between the urokinase plasminogen activator (uPA) system and the leukemia bone marrow microenvironment (BMM). We established BMSCs-HL60 and HS-5-K562 co-culture models in direct contact mode to simulate the BMM in leukemia. In BMSCs-HL60 co-culture model, the expression levels of uPA, uPA receptor (uPAR), plasminogen activator inhibitor 1 (PAI-1) and vascular endothelial growth factor (VEGF) in BMSCs were higher than those in mono-cultured BMSCs. Matrix metalloproteinase (MMP)-9 (MMP-9) was up-regulated in co-cultured HL60 cells. In HS-5-K562 co-culture model, only uPA, PAI-1, and VEGF-A were up-regulated in HS-5 cells. The levels of the uPA protein in the co-culture supernatant were significantly higher than that of mono-cultured BMSCs or HS-5 cells. Our findings demonstrate that the co-culture stimulates the production of uPA, uPAR, PAI-1, MMP-9, and VEGF-A by BMSCs. It could further explain how the uPA system in leukemia cells is involved in the growth, development, and prognosis of leukemia.

Indexed as

Cell CommunicationAdolescentAdultChildFemaleHL-60 CellsHumansK562 CellsLeukemiaMaleMatrix Metalloproteinase 9Membrane ProteinsMesenchymal Stem CellsMiddle AgedPlasminogen Activator Inhibitor 1Receptors, Urokinase Plasminogen ActivatorMatrix Metalloproteinase 9Membrane ProteinsMMP9 protein, humanPlasminogen Activator Inhibitor 1PLAU protein, humanPLAUR protein, humanReceptors, Urokinase Plasminogen ActivatorSERPINE1 protein, humanVascular Endothelial Growth Factor AVEGFA protein, humanHL60HS-5K562matrix metalloproteins-9vascular endothelial growth factor

Identifiers

PMID33146708
PMCPMC7677749
OpenAlexW3097915526

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.