ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2020
Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A safe and efficacious inactivated vaccine aids prevent reproductive failure associated with congenital toxoplasmosis in ovine.Veterinary research · 2026Article
- Quantitative Risk Assessment of Oocyst Versus Bradyzoite Foodborne Transmission ofPathogens (Basel, Switzerland) · 2023Article
- Sarcocyst Quantification and Viability: Freezing Treatment as an Alternative to Carcass Condemnation.Acta parasitologica · 2023Article
- Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks withMicroorganisms · 2022Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium monoazide (PMA). The method was called qPCR-PMA. Oocyst aliquots were incubated (15 min) at 25 ºC or 100 ºC and analyzed by microscopy, after trypan blue and neutral red staining. Microscopic investigation determined viable and non-viable oocysts. For the molecular investigation, both aliquots of oocysts were treated with PMA. Non-viable oocysts, after PMA treatment, exhibited an inhibition of DNA amplification by qPCR. Although analyses were carried out with oocysts treated experimentally, these results suggest that qPCR-PMA can be a useful strategy to distinguish viable and non-viable T. gondii oocysts in water safety testing, showing if water is safe to drink.
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Registered trials
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