Evidence map›Paper›PMID 33145960›Full record

ArticleStem cells translational medicine2021

Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix.

Reena Singh, Louise Cottle, Thomas Loudovaris, Di Xiao, Pengyi Yang, Helen E Thomas, Melkam A Kebede, Peter Thorn

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 28 citations in OpenAlex.

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  9. Benefits and Hurdles of Pancreatic β-Cell Replacement.Stem cells translational medicine · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Reena SinghCharles Perkins Centre, Discipline of Physiology, School of Medical Sciences, University of Sydney, Camperdown, New South Wales, Australia.ORCID 0000-0001-8860-8800
Louise CottleCharles Perkins Centre, Discipline of Physiology, School of Medical Sciences, University of Sydney, Camperdown, New South Wales, Australia.
Thomas LoudovarisSt Vincent's Institute, Fitzroy, Victoria, Australia.
Di XiaoComputational Systems Biology Group, Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia.
Pengyi YangComputational Systems Biology Group, Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia.
Helen E ThomasSt Vincent's Institute, Fitzroy, Victoria, Australia.
Melkam A KebedeCharles Perkins Centre, Discipline of Physiology, School of Medical Sciences, University of Sydney, Camperdown, New South Wales, Australia.
Peter ThornCharles Perkins Centre, Discipline of Physiology, School of Medical Sciences, University of Sydney, Camperdown, New South Wales, Australia.ORCID 0000-0002-3228-770X
University of Sydney · AUChildren's Medical Research Institute · AUSt Vincent's Hospital · AUSt Vincents Institute of Medical Research · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The differentiation of human stem cells into insulin secreting beta-like cells holds great promise to treat diabetes. Current protocols drive stem cells through stages of directed differentiation and maturation and produce cells that secrete insulin in response to glucose. Further refinements are now needed to faithfully phenocopy the responses of normal beta cells. A critical factor in normal beta cell behavior is the islet microenvironment which plays a central role in beta cell survival, proliferation, gene expression and secretion. One important influence on native cell responses is the capillary basement membrane. In adult islets, each beta cell makes a point of contact with basement membrane protein secreted by vascular endothelial cells resulting in structural and functional polarization. Interaction with basement membrane proteins triggers local activation of focal adhesions, cell orientation, and targeting of insulin secretion. This study aims to identifying the role of basement membrane proteins on the structure and function of human embryonic stem cell and induced pluripotent stem cell-derived beta cells. Here, we show that differentiated human stem cells-derived spheroids do contain basement membrane proteins as a diffuse web-like structure. However, the beta-like cells within the spheroid do not polarize in response to this basement membrane. We demonstrate that 2D culture of the differentiated beta cells on to basement membrane proteins enforces cell polarity and favorably alters glucose dependent insulin secretion.

Indexed as

Extracellular MatrixInsulin-Secreting CellsPluripotent Stem CellsCell DifferentiationEndothelial CellsGlucoseHumansInsulinGlucoseInsulinbasement membranediabetesdifferentiationglucose stimulated insulin secretionhuman pluripotent stem cell-derived beta cells

Identifiers

PMID33145960
PMCPMC7900592
OpenAlexW3095735543

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.