ArticleACS biomaterials science & engineering2020
SpheroidChip: Patterned Agarose Microwell Compartments Harboring HepG2 Spheroids are Compatible with Genotoxicity Testing.
Article in ACS biomaterials science & engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- High-Throughput Generation of Tumor Spheroids via Droplet Microfluidics for siRNA-Loaded Nanomedicine Assessment.Advanced healthcare materials · 2026Article
- Index matching improves the imaging quality of 3D well-of-the-well dishes for embryo culture.Biophotonics discovery · 2026Article
- Influence of Structure-Property Relationships of Polymeric Biomaterials for Engineering Multicellular Spheroids.Bioengineering (Basel, Switzerland) · 2025Review
- A Novel Microfluidic Platform for Personalized Anticancer Drug Screening Through Image Analysis.Micromachines · 2024Article
- Microfluidic Droplet-Assisted Fabrication of Vessel-Supported Tumors for Preclinical Drug Discovery.ACS applied materials & interfaces · 2023Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Liver-on-a-chip: Considerations, advances, and beyond.Biomicrofluidics · 2022Review
- Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip.Cell reports methods · 2022Article
- Natural Biopolymers as Additional Tools for Cell Microencapsulation Applied to Cellular Therapy.Polymers · 2022Review
- Article
- Human Variation in DNA Repair, Immune Function, and Cancer Risk.Frontiers in immunology · 2022Review
- Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues.Biophysical reviews · 2021Review
- Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment.International journal of bioprinting · 2021Review
- Engineered Microsystems for Spheroid and Organoid Studies.Advanced healthcare materials · 2021Review
- Review
- Applications of CometChip for Environmental Health Studies.Chemical research in toxicology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Three-dimensional tissue culture models are emerging as effective alternatives to animal testing. They are especially beneficial for liver toxicity studies, enabling hepatocytes to display improved levels of liver-specific functions. One common model is hepatocyte spheroids, which are spontaneously formed cell aggregates. Techniques for spheroid formation include the use of ultralow attachment plates and the hanging drop method, both of which are technically challenging and relatively low throughput. Here, we describe a simple-to-use platform that improves spheroid production and is compatible with genotoxicity testing by the comet assay. To achieve this, we created a chip containing a microwell array where dozens of spheroids are contained within a single well of a 96-well plate. The microwells are made from agarose, a nontoxic material suitable for cell growth and spheroid formation. HepG2 cells loaded into customizable microwells formed spheroids through agarose-assisted aggregation within one to two days. In addition, the agarose matrix allows the same platform to be used in DNA damage analysis. Specifically, the comet assay enables quantification of DNA breaks based on the increased migration of damaged DNA through agarose during electrophoresis. Here, we developed a modified comet assay and show that intact HepG2 spheroids cultured in microwells can be electrophoresed to reveal the extent of DNA damage following exposure to inflammatory chemicals (H
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.