ArticleJournal of thoracic disease2020
Comparison of the next-generation sequencing (NGS) technology with culture methods in the diagnosis of bacterial and fungal infections.
Article in Journal of thoracic disease, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Metagenomics next-generation sequencing for the diagnosis of central nervous system infection: A systematic review and meta-analysis.Frontiers in neurology · 2022Pooled it
- The Value of Macrogene Second-Generation Sequencing in the Diagnosis, Guidance of Drug Use, and Efficacy Monitoring of Infectious Pneumonia in Premature Infants.Computational and mathematical methods in medicine · 2022Trial
- Characterization of the Healthy Ocular Mycobiome of Purebred Lusitano Horses Using Oxford Nanopore Long-Read Sequencing.Animals : an open access journal from MDPI · 2026Article
- The Impact of Vaginal Bacteria and Antimicrobial Treatment on Pregnancy Outcomes in Healthy Breeding Bitches.Antibiotics (Basel, Switzerland) · 2026Article
- Next-generation sequencing: Driving a paradigm shift from pathogen detection to systems-level analysis.Infectious medicine · 2026Article
- Bacteria and Fungi Synergistically Reprogram Flavonoid Metabolites in the Pericarp of Citrus Reticulata 'Chachi' During Storage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Pseudoaneurysm of the abdominal aorta due to brucella infection: Next-generation sequencing diagnosis and endovascular treatment.Journal of vascular surgery cases and innovative techniques · 2026Article
- Comprehensive analysis of microorganisms in severe septic patients in the intensive care unit by mNGS and microbial culture.BMC microbiology · 2026Article
- Misdiagnosis of invasive pulmonary fungal disease caused by drug-resistantFrontiers in medicine · 2026Article
- Metagenomic next-generation sequencing unveils invasive aspergillosis masquerading as miliary tuberculosis in a neutropenic leukemia patient: a case report.Frontiers in fungal biology · 2026Article
- The gastrointestinal microbiome in critical illness: A Clinician's guide to mechanisms, emerging tools, and therapeutic questions.Critical care and resuscitation : journal of the Australasian Academy of Critical Care Medicine · 2025Review
- Microbial community of civet excreta fed by robusta cherry coffee in Indonesian civet coffee production.Journal of advanced veterinary and animal research · 2025Article
- A rareFrontiers in cellular and infection microbiology · 2025Article
- Study on the application of microfluidic-based in vitro diagnostic technology in pathogenic detection of respiratory tract infections.Journal of translational medicine · 2024Article
- The shoulder microbiome: a systematic review and meta analysis.JSES reviews, reports, and techniques · 2024Review
- Fungal lung disease.The European respiratory journal · 2024Review
- Utilizing Next-Generation Sequencing: Advancements in the Diagnosis of Fungal Infections.Diagnostics (Basel, Switzerland) · 2024Review
- Article
- Comparison of metagenomic next-generation sequencing and conventional culture for the diagnostic performance in febrile patients with suspected infections.BMC infectious diseases · 2024Article
- Culture Negative Pasteurella multocida Confirmed Prosthetic Hip Infection using Next-generation Sequencing.Journal of orthopaedic case reports · 2024Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial and fungal infections that caused by various kinds of pathogens are frequently-occurring disease all over the world. To overcome the shortcomings of traditional culture method, we have adapted next-generation sequencing (NGS) technology to identify pathogens.
methodsIn this study, clinical samples from 20 patients pre-diagnosed with bacterial and fungal infections in the Shanghai Pulmonary Hospital of Tongji University, China, were investigated retrospectively to compare the NGS with conventional "gold standard" culture methods.
resultsThe detection rates of bacterial or fungal infections were 95.0% (19/20) by NGS and 60.0% (12/20) by culture method. There was a significant difference between the results of NGS and traditional culture method by using McNemar's χ
conclusionsNGS, as an emerging diagnostic technology, shows outstanding advantages in the diagnosis of bacterial and fungal infections, and optimizes the treatment of infectious diseases. The clinical application and future development of NGS technology are worthy of expectation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.