ArticleScientific reports2020
Rampant loss of social traits during domestication of a Bacillus subtilis natural isolate.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Root microbes can improve plant tolerance to insect damage: A systematic review and meta-analysis.Ecology · 2025Pooled it
- Toward a Functional Genetics of Adaptation: Insights From Microbial Experimental Evolution.Genome biology and evolution · 2026Review
- Disruption of bacteriophage integration site promotes rapid diversification of multicellular traits inMicrobiology spectrum · 2026Article
- Glutamate facilitates root colonization by plant growth-promoting rhizobacteriaMicrobiology spectrum · 2026Article
- Lives in a gram of soil: linking life cycle biology to soil ecology.The ISME journal · 2026Review
- Toward food-grade production of the Bacteroides helcogenes protein-glutamine glutaminase with an optimized Bacillus subtilis strain.Applied microbiology and biotechnology · 2026Article
- Genome evolution ofApplied and environmental microbiology · 2025Article
- Presence of a biofilm beneficiary alters the evolutionary trajectory of a biofilm former.The ISME journal · 2025Article
- Enhanced surface colonisation and competition during bacterial adaptation to a fungus.Nature communications · 2024Article
- Synergistic phenotypic adaptations of motile purple sulphur bacteria Chromatium okenii during lake-to-laboratory domestication.PloS one · 2024Article
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- Intrahost evolution of the gut microbiota.Nature reviews. Microbiology · 2023Review
- Attempts to limit sporulation in the probiotic strainAccess microbiology · 2023Article
- Article
- Adaptation to novel spatially-structured environments is driven by the capsule and alters virulence-associated traits.Nature communications · 2022Article
- Insights in the Complex DegU, DegS, and Spo0A Regulation System of Paenibacillus polymyxa by CRISPR-Cas9-Based Targeted Point Mutations.Applied and environmental microbiology · 2022Article
- Improved Mobilome Delineation in Fragmented Genomes.Frontiers in bioinformatics · 2022Article
- Analysis of the Phospholipid Profile of the Collection Strain PAO1 and Clinical Isolates ofInternational journal of molecular sciences · 2021Article
- A Seed-EndophyticFrontiers in microbiology · 2021Article
- Mechanism Across Scales: A Holistic Modeling Framework Integrating Laboratory and Field Studies for Microbial Ecology.Frontiers in microbiology · 2021Article
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Authors and funding
4 authors.
Funding
Abstract
Most model bacteria have been domesticated in laboratory conditions. Yet, the tempo with which a natural isolate diverges from its ancestral phenotype under domestication to a novel laboratory environment is poorly understood. Such knowledge, however is essential to understanding the rate of evolution, the time scale over which a natural isolate can be propagated without loss of its natural adaptive traits, and the reliability of experimental results across labs. Using experimental evolution, phenotypic assays, and whole-genome sequencing, we show that within a week of propagation in a common laboratory environment, a natural isolate of Bacillus subtilis acquires mutations that cause changes in a multitude of traits. A single adaptive mutational step in the gene coding for the transcriptional regulator DegU impairs a DegU-dependent positive autoregulatory loop and leads to loss of robust biofilm architecture, impaired swarming motility, reduced secretion of exoproteases, and to changes in the dynamics of sporulation across environments. Importantly, domestication also resulted in improved survival when the bacteria face pressure from cells of the innate immune system. These results show that degU is a target for mutations during domestication and underscores the importance of performing careful and extremely short-term propagations of natural isolates to conserve the traits encoded in their original genomes.
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