ArticleNature communications2020
BrainPhys neuronal medium optimized for imaging and optogenetics in vitro.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Modelling synaptic dysfunction in childhood dementia using human iPSC-derived cortical networks.Nature communications · 2026Article
- Leveraging AI for cell biology discovery.Biochemical Society transactions · 2026Review
- A splice-switching antisense oligonucleotide approach for pediatric genetic epilepsies.bioRxiv : the preprint server for biology · 2025Article
- Optimizing the in vitro neuronal microenvironment to mitigate phototoxicity in live-cell imaging.Stem cell research & therapy · 2025Article
- The Concomitant Effect of the Antiepileptic Drug Lacosamide and rTMS on an SH-SY5Y Model of Neuronal Excitability.Neurology international · 2025Article
- Astrocytes contribute to toll-like receptor 2-mediated neurodegeneration and alpha-synuclein pathology in a human midbrain Parkinson's model.Translational neurodegeneration · 2024Article
- Revealing single-neuron and network-activity interaction by combining high-density microelectrode array and optogenetics.Nature communications · 2024Article
- Optogenetics in Pancreatic Islets: Actuators and Effects.Diabetes · 2024Review
- Human iPSC-Derived Neurons with Reliable Synapses and Large Presynaptic Action Potentials.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Recent advances and applications of human brain models.Frontiers in neural circuits · 2024Review
- Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion.Journal of the American Chemical Society · 2023Article
- Human cerebrospinal fluid affects chemoradiotherapy sensitivities in tumor cells from patients with glioblastoma.Science advances · 2023Article
- Differentiation, Transcriptomic Profiling, and Calcium Imaging of Human Hypothalamic Neurons.Current protocols · 2023Article
- Mapping PTBP2 binding in human brain identifies SYNGAP1 as a target for therapeutic splice switching.Nature communications · 2023Article
- Druggable transcriptomic pathways revealed in Parkinson's patient-derived midbrain neurons.NPJ Parkinson's disease · 2022Article
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Authors and funding
18 authors.
Funding
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Abstract
The capabilities of imaging technologies, fluorescent sensors, and optogenetics tools for cell biology are advancing. In parallel, cellular reprogramming and organoid engineering are expanding the use of human neuronal models in vitro. This creates an increasing need for tissue culture conditions better adapted to live-cell imaging. Here, we identify multiple caveats of traditional media when used for live imaging and functional assays on neuronal cultures (i.e., suboptimal fluorescence signals, phototoxicity, and unphysiological neuronal activity). To overcome these issues, we develop a neuromedium called BrainPhys™ Imaging (BPI) in which we optimize the concentrations of fluorescent and phototoxic compounds. BPI is based on the formulation of the original BrainPhys medium. We benchmark available neuronal media and show that BPI enhances fluorescence signals, reduces phototoxicity and optimally supports the electrical and synaptic activity of neurons in culture. We also show the superior capacity of BPI for optogenetics and calcium imaging of human neurons. Altogether, our study shows that BPI improves the quality of a wide range of fluorescence imaging applications with live neurons in vitro while supporting optimal neuronal viability and function.
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