Evidence map›Paper›PMID 33144418›Full record

ArticleIn vivo (Athens, Greece)

Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models.

Hiroto Nishino, Hannah M Hollandsworth, Norihiko Sugisawa, Jun Yamamoto, Yoshihiko Tashiro, Sachiko Inubushi, Kazuyuki Hamada, Y U Sun, Hyein Lim, Siamak Amirfakhri and 3 more

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Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hiroto NishinoDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Hannah M HollandsworthDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Norihiko SugisawaDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Jun YamamotoDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Yoshihiko TashiroDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Sachiko InubushiDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Kazuyuki HamadaDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Y U SunDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Hyein LimDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Siamak AmirfakhriDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Filemoni FilemoniDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Robert M HoffmanDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A.
Michael BouvetDepartment of Surgery, University of California San Diego, San Diego, CA, U.S.A. mbouvet@ucsd.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimSurgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. MATERIALS AND

methodsHuman HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis.

resultsSix weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm

conclusionThis novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.

Indexed as

Carcinoma, HepatocellularLiver NeoplasmsAnimalsDisease Models, AnimalHumansMiceMice, NudeNeoplasm Transplantationcell linecolon cancerHepatocellular carcinomaliver metastasisnude mouseorthotopicpatient tumorPDOX

Identifiers

PMID33144418
PMCPMC7811661

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.