ArticleInternational journal of molecular sciences2020
The Extent of Intracellular Accumulation of Bilirubin Determines Its Anti- or Pro-Oxidant Effect.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- The Nonlinear Relationship Between Total Bilirubin and Coronary Heart Disease: A Dose-Response Meta-Analysis.Frontiers in cardiovascular medicine · 2021Pooled it
- Chitosan Nanoparticles Unlock the Antioxidant Potential of Epigallocatechin Gallate in Pancreatic and Hepatic Cancer Cell Models.ACS omega · 2026Article
- The Role of ER Stress in Bilirubin Neurotoxicity: A Complex Molecular Network.Antioxidants (Basel, Switzerland) · 2026Review
- Heme in Bacterial Pathogenesis and as an Antimicrobial Target.Chemical reviews · 2025Review
- Unbound bilirubin and risk of severe neurodevelopmental impairment in extremely low birthweight newborns.Pediatric research · 2025Article
- Association of High-Sensitivity Cardiac Troponin I, Platelet Counts, and Total Bilirubin/Albumin Ratio with the Severity of Neonatal Hyperbilirubinemia.Physiological research · 2025Article
- The diagnostic potential of unconjugated bilirubin in schizophrenia and bipolar disorder.BMC psychiatry · 2025Article
- Transcriptomic and Proteomic Changes in the Brain Along with Increasing Phenotypic Severity in a Rat Model of Neonatal Hyperbilirubinemia.International journal of molecular sciences · 2025Article
- Further insights into influence factors of hypertension in older patients with obstructive sleep apnea syndrome: a model based on multiple centers.Aging clinical and experimental research · 2025Article
- Albumin-bilirubin score: a promising predictor of postoperative distant metastasis in patients with colorectal cancer.Biomarkers in medicine · 2025Article
- The Protective Effect of Bilirubin on MAFLD May Be Mediated by Improving Insulin Re-Sistance and Alleviating Chronic Inflammation.Journal of inflammation research · 2025Article
- Molecular events in brain bilirubin toxicity revisited.Pediatric research · 2024Review
- Article
- Antioxidant Activity of Bilirubin in Micellar and Liposomal Systems Is pH-Dependent.Antioxidants (Basel, Switzerland) · 2024Article
- Association of Different Total Bilirubin Levels with Prognosis of Peritoneal Dialysis-Associated Peritonitis.Medicina (Kaunas, Lithuania) · 2023Article
- [Nonalcoholic fatty liver disease and bilirubin: correlation, mechanism, and therapeutic perspectives].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2023Article
- High indirect bilirubin levels as an independent predictor of postoperative myasthenic crisis: a single-center, retrospective study.Frontiers in neurology · 2023Article
- The J-shape Association between Total Bilirubin and Stroke in Older Patients with Obstructive Sleep Apnea Syndrome: A Multicenter Study.The journal of nutrition, health & aging · 2023Article
- Bile Duct Ligation Impairs Function and Expression of Mrp1 at Rat Blood-Retinal Barrier via Bilirubin-Induced P38 MAPK Pathway Activations.International journal of molecular sciences · 2022Article
- Translational Approach to the Protective Effect of Bilirubin in Diabetic Kidney Disease.Biomedicines · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundSevere hyperbilirubinemia can cause permanent neurological damage in particular in neonates, whereas mildly elevated serum bilirubin protects from various oxidative stress-mediated diseases. The present work aimed to establish the intracellular unconjugated bilirubin concentrations (iUCB) thresholds differentiating between anti- and pro-oxidant effects.
methodsHepatic (HepG2), heart endothelial (H5V), kidney tubular (HK2) and neuronal (SH-SY5Y) cell lines were exposed to increasing concentration of bilirubin. iUCB, cytotoxicity, intracellular reactive oxygen species (ROS) concentrations, and antioxidant capacity (50% efficacy concentration (EC
resultsExposure of SH-SY5Y to UCB concentration > 3.6 µM (iUCB of 25 ng/mg) and >15 µM in H5V and HK2 cells (iUCB of 40 ng/mg) increased intracellular ROS production (
conclusionsIn all the cell lines studied, iUCB of around 7 ng/mg protein had antioxidant activities, while iUCB > 25 ng/mg protein resulted in a prooxidant and cytotoxic effects. UCB metabolism was found to be cell-specific resulting in different iUCB.
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