Evidence map›Paper›PMID 33143040›Full record

ArticleJournal of fungi (Basel, Switzerland)2020

Bringing SEM and MSI Closer Than Ever Before: Visualizing

Tereza Juříková, Dominika Luptáková, Olga Kofroňová, Anton Škríba, Jiří Novák, Helena Marešová, Andrea Palyzová, Miloš Petřík, Vladimír Havlíček, Oldřich Benada

Open access · goldAbstract read
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Omics for deciphering oral microecology.International journal of oral science · 2024
    Review
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Tereza JuříkováInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0003-2836-0393
Dominika LuptákováInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0002-3691-2584
Olga KofroňováInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0002-5435-4088
Anton ŠkríbaInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0003-1122-1098
Jiří NovákInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0003-1902-4304
Helena MarešováInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0003-2462-390X
Andrea PalyzováInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0003-0852-9841
Miloš PetříkInstitute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic.ORCID 0000-0003-1334-5916
Vladimír HavlíčekInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0002-8614-7059
Oldřich BenadaInstitute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, 142 20 Prague, Czech Republic.ORCID 0000-0002-4592-6216
Czech Academy of Sciences · CZInstitute of Molecular and Translational Medicine · CZ

Funding

Czech Science Foundation 19-10907SMinistry of Education, Youth and Sports of the Czech Republic LO1509
6 · The paper itself

Abstract

A procedure for processing frozen rat lung tissue sections for scanning electron microscopy (SEM) from deeply frozen samples initially collected and stored for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed. The procedure employed slow thawing of the frozen sections while floating on the surface and melting in a fixative solution. After the float-washing step, the sections were dehydrated in a graded ethanol series and dried in a critical point dryer. The SEM generated images with well-preserved structures, allowing for monitoring of bacterial cells and fungal hyphae in the infected tissue. Importantly, the consecutive nonfixed frozen sections were fully compatible with MALDI-MSI, providing molecular biomarker maps of

Indexed as

bacteriafixationfungimatrix-assisted laser desorption/ionization mass spectrometry imagingrat lung tissuescanning electron microscopy

Identifiers

PMID33143040
PMCPMC7711807
OpenAlexW3096159130

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.