Evidence map›Paper›PMID 33142729›Full record

ArticleInternational journal of molecular sciences2020

Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites.

Christian Kleusch, Cornelia Monzel, Krishna Chander Sridhar, Bernd Hoffmann, Agnes Csiszár, Rudolf Merkel

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Synthetic Lipid Biology.Chemical reviews · 2025
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Christian KleuschInstitute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
Cornelia MonzelInstitute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
Krishna Chander SridharInstitute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
Bernd HoffmannInstitute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
Agnes CsiszárInstitute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
Rudolf MerkelInstitute of Biological Information Processing, IBI-2: Mechanobiology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.ORCID 0000-0003-3178-3282

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cells adhere to the extracellular matrix at distinct anchoring points, mostly focal adhesions. These are rich in immobile transmembrane- and cytoskeletal-associated proteins, some of which are known to interact with lipids of the plasma membrane. To investigate their effect on lipid mobility and molecular interactions, fluorescently labeled lipids were incorporated into the plasma membranes of primary myofibroblasts using fusogenic liposomes. With fluorescence correlation spectroscopy, we tested mobilities of labeled microdomain-associated lipids such as sphingomyelin (SM), ganglioside (GM1), and cholesterol as well as of a microdomain-excluded phospholipid (PC) and a lipid-like molecule (DiIC

Indexed as

Focal AdhesionsAnimalsCell MembraneCells, CulturedCholesterolEmbryo, MammalianFluorescenceLipid BilayersLipidsMembrane MicrodomainsMyofibroblastsRatsRats, WistarSpectrometry, FluorescenceCholesterolLipid BilayersLipidsfluorescence correlation spectroscopy (FCS)focal adhesion sitefusogenic liposomeslipid diffusionliquid ordered and disordered phasesmembrane microdomainsmodel membrane

Identifiers

PMID33142729
PMCPMC7662714

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.