Evidence map›Paper›PMID 33141355›Full record

ArticleMikrochimica acta2020

Impedimetric DNA E-biosensor for multiplexed sensing of Escherichia coli and its virulent f17 strains.

Amal Rabti, Riham Zayani, Marwa Meftah, Imed Salhi, Noureddine Raouafi

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Recent Advancements in Technologies to Detect EnterohaemorrhagicJournal of microbiology and biotechnology · 2023
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Amal RabtiSensors and Biosensors Group, Analytical Chemistry and Electrochemistry Lab (LR99ES15), Faculty of Science, University of Tunis El Manar, 2092, Tunis El Manar, Tunisia.
Riham ZayaniSensors and Biosensors Group, Analytical Chemistry and Electrochemistry Lab (LR99ES15), Faculty of Science, University of Tunis El Manar, 2092, Tunis El Manar, Tunisia.
Marwa MeftahSensors and Biosensors Group, Analytical Chemistry and Electrochemistry Lab (LR99ES15), Faculty of Science, University of Tunis El Manar, 2092, Tunis El Manar, Tunisia.
Imed SalhiLivestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute (I.R.A), University of Gabès, Medenine, Tunisia.
Noureddine RaouafiSensors and Biosensors Group, Analytical Chemistry and Electrochemistry Lab (LR99ES15), Faculty of Science, University of Tunis El Manar, 2092, Tunis El Manar, Tunisia. noureddine.raouafi@fst.utm.tn.ORCID 0000-0001-8938-8221
Tunis El Manar University · TNInstitut des Régions Arides · TN

Funding

Ministère de l'Enseignement Supérieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communication PRF2017-D4P1Ministère de l'Enseignement Supérieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communication PRFCOV-D2P2Ministère de l'Enseignement Supérieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communication PRVCOV19-D3P1
6 · The paper itself

Abstract

An impedance-based DNA multiplexed biosensor was designed to simultaneously detect Escherichia coli (yaiO gene) and its virulent f17 variant. The thiolated DNA dual probe was self-assembled onto the surface of the gold nanoparticle-modified screen-printed carbon electrode (AuNPs/SPCE) to recognize selected sequences from yaiO and f17 genes. The optimal conditions to prepare the bioelectrode were determined based on the monitoring of the impedimetric response fitted to an equivalent electrical circuit model. The charge transfer resistance of the bioelectrode increased by recognizing the target DNA sequences. The limit of detection was 0.8 fM and 1.0 fM for yaiO and f17 target DNA, respectively, and the linearity ranged from 1 × 10

Indexed as

Biosensing TechniquesDNA, BacterialElectric ImpedanceElectrochemical TechniquesEscherichia coliGoldMetal NanoparticlesVirulenceDNA, BacterialGoldDual biosensorEscherichia coliF17 fimbriaeImpedance spectroscopyMultiplexingPathogen

Identifiers

PMID33141355
OpenAlexW3095352109

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.