Evidence map›Paper›PMID 33141339›Full record

ArticleGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association2021

Molecular alterations in gastric cancer and the surrounding intestinal metaplastic mucosa: an analysis of isolated glands.

Ryo Sugimoto, Wataru Habano, Naoki Yanagawa, Risaburo Akasaka, Yosuke Toya, Akira Sasaki, Takayuki Matsumoto, Tamotsu Sugai

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Gastric epithelial histology and precancerous conditions.World journal of gastrointestinal oncology · 2022
    Review
  5. Article
  6. Article
  7. Aberrant DNA methylation and expression ofSaudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Ryo SugimotoDepartment of Molecular Diagnostic Pathology, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Wataru HabanoDepartment of Pharmacodynamics and Molecular Genetics, School of Pharmacy, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Naoki YanagawaDepartment of Molecular Diagnostic Pathology, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Risaburo AkasakaDivision of Gastroenterology, Department of Internal Medicine, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Yosuke ToyaDivision of Gastroenterology, Department of Internal Medicine, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Akira SasakiDepartment of Surgery, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Takayuki MatsumotoDivision of Gastroenterology, Department of Internal Medicine, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan.
Tamotsu SugaiDepartment of Molecular Diagnostic Pathology, School of Medicine, Iwate Medical University, 2-1-1, Shiwa, Yahaba, Morioka, 028-3695, Japan. tsugai@iwate-med.ac.jp.ORCID http://orcid.org/0000-0002-4896-3557
Iwate Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIntestinal metaplasias (IMs) are generally regarded as pre-neoplastic gastric lesions. However, molecular alterations including genetic and epigenetic changes occurring in individual IM glands are not well defined.

aimsWe sought to identify DNA methylation status, microsatellite instability (MSI) and allelic imbalance (AI) occurring in individual IM glands and non-IM glands within the same mucosa.

methodsWe divided examined isolated gland obtained from GC into 4 components: isolated cancer, antral isolated intestinal metaplastic tissue, antral isolated non-metaplastic gland and isolated non-metaplastic gland derived from the greater curvature of the most distant gastric body without mucosal atrophy. We examined AI and microsatellite instability statuses using PCR-based microsatellite analysis. Next, the DNA methylation status (high methylation epigenome [HME], intermediate methylation epigenome [IME], and low methylation epigenome [LME]) was investigated. DNA methylation analysis of CDKN2A, mir34-b/c and MLHI genes was also performed.

resultsAlthough antral isolated IM glands were characterized by IME, isolated non-IM glands showed LME. In isolated cancer glands, HME was frequently found, compared with isolated non-IM glands. DNA methylation of mir34-b/c was common in isolated cancer and IM glands, whereas DNA methylation of CDKN2A was a rare event in isolated samples. The MLH1 gene was not methylated in isolated non-IM glands. Although multiple AIs were frequently found in isolated cancer glands, a few AIs were detected in isolated IM glands.

conclusionsWe suggest that the DNA methylation status and the status of the mir34-b/c gene among isolated samples of IMs and isolated non-IM glands have an impact on IM development.

Indexed as

Microsatellite InstabilityAgedAged, 80 and overAllelic ImbalanceDNA MethylationEpigenesis, GeneticFemaleHumansIntestinal MucosaMaleMetaplasiaMiddle AgedStomach NeoplasmsCrypt isolation methodDNA methylationGastric cancerIntestinal metaplasiaLoss of heterozygosity

Identifiers

PMID33141339
OpenAlexW3095894580

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.