ArticleEMBO reports2020
Single-cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland.
Article in EMBO reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Resolving thyroid lineage cell trajectories merging into a dual endocrine gland in mammals.Nature communications · 2026Article
- Zebrafish: a key model for unraveling endocrine-disrupting effects on thyroid development and function.Frontiers in pharmacology · 2026Review
- Single-cell analysis reveals the cellular and transcriptional heterogeneity of the normal pediatric thyroid.Frontiers in endocrinology · 2026Article
- Multiomics Investigation of Exhausted T Cells in Glioblastoma Tumor Microenvironment:International journal of molecular sciences · 2025Article
- Acquisition of neural crest promoted thyroid evolution from chordate endostyle.Science advances · 2025Article
- Identifying optimal conditions for precise knock-in of exogenous DNA into the zebrafish genome.Development (Cambridge, England) · 2025Article
- Mutations in the bone morphogenetic protein signaling pathway sensitize zebrafish and humans to ethanol-induced jaw malformations.Disease models & mechanisms · 2025Article
- Tumor Cell Plasticity and Stromal Microenvironment Distinguish Papillary and Follicular Growth Patterns in a Mouse Model of BRAFV600E-Induced Thyroid Cancer.Cancer research communications · 2025Article
- Experimentally Manipulating the Thyroid Hormone Axis in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Compromised COPII vesicle trafficking leads to glycogenic hepatopathy.Disease models & mechanisms · 2024Article
- Deficiency of the HGF/Met pathway leads to thyroid dysgenesis by impeding late thyroid expansion.Nature communications · 2024Article
- Spatially resolved single-cell atlas of ascidian endostyle provides insight into the origin of vertebrate pharyngeal organs.Science advances · 2024Article
- Fishing Innate Immune System Properties through the Transcriptomic Single-Cell Data ofBiology · 2023Review
- Notch signaling in thyrocytes is essential for adult thyroid function and mammalian homeostasis.Nature metabolism · 2023Article
- The thyroid gland under Notch control.Nature metabolism · 2023Article
- Clinical significance of extrathyroidal extension to major vessels in papillary thyroid carcinoma.Journal of endocrinological investigation · 2023Article
- Efficient knock-in method enabling lineage tracing in zebrafish.Life science alliance · 2023Article
- Transcriptional Analysis of the Endostyle Reveals Pharyngeal Organ Functions in Ascidian.Biology · 2023Article
- Modeling Human Thyroid Development by Fetal Tissue-Derived Organoid Culture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Cryo-EM: A new dawn in thyroid biology.Molecular and cellular endocrinology · 2021Review
Corrections and comments
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Authors and funding
19 authors at 3 institutions in 2 countries.
Funding
Abstract
The thyroid gland regulates growth and metabolism via production of thyroid hormone in follicles composed of thyrocytes. So far, thyrocytes have been assumed to be a homogenous population. To uncover heterogeneity in the thyrocyte population and molecularly characterize the non-thyrocyte cells surrounding the follicle, we developed a single-cell transcriptome atlas of the region containing the zebrafish thyroid gland. The 6249-cell atlas includes profiles of thyrocytes, blood vessels, lymphatic vessels, immune cells, and fibroblasts. Further, the thyrocytes show expression heterogeneity, including bimodal expression of the transcription factor pax2a. To validate thyrocyte heterogeneity, we generated a CRISPR/Cas9-based pax2a knock-in line that monitors pax2a expression in the thyrocytes. A population of pax2a-low mature thyrocytes interspersed in individual follicles can be distinguished. We corroborate heterogeneity within the thyrocyte population using RNA sequencing of pax2a-high and pax2a-low thyrocytes, which demonstrates 20% differential expression in transcriptome between the two subpopulations. Our results identify and validate transcriptional differences within the presumed homogenous thyrocyte population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.