ArticleJournal of anatomy2021
A simple nuclear contrast staining method for microCT-based 3D histology using lead(II) acetate.
Article in Journal of anatomy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Quantitative Stain Mapping in X-Ray Virtual Histology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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- Unveiling Another Dimension: Advanced Visualization of Cancer Invasion and Metastasis via Micro-CT Imaging.Cancers · 2025Article
- Contrast-Enhanced Micro-CT Imaging of Murine Mandibles: A Multi-Method Approach for Simultaneous Hard and Soft Tissue Analysis.Journal of clinical periodontology · 2025Article
- In situ isotropic 3D imaging of vasculature perfusion specimens using x-ray microscopic dual-energy CT.Journal of microscopy · 2025Article
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- Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT.Journal of medical imaging (Bellingham, Wash.) · 2024Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
- Three-Dimensional Cell Culture Micro-CT Visualization within Collagen Scaffolds in an Aqueous Environment.Cells · 2024Article
- Chondrosarcoma evaluation using hematein-based x-ray staining and high-resolution 3D micro-CT: a feasibility study.European radiology experimental · 2024Article
- Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea.Heliyon · 2024Article
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- Gene expression detection in developing mouse tissue using in situ hybridization and µCT imaging.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling.Journal of cardiovascular development and disease · 2022Article
- Micro-CT in ophthalmology:Quantitative imaging in medicine and surgery · 2022Article
- Mouse embryo phenotyping using X-ray microCT.Frontiers in cell and developmental biology · 2022Review
- Elastic transformation of histological slices allows precise co-registration with microCT data sets for a refined virtual histology approach.Scientific reports · 2021Article
- A simple nuclear contrast staining method for microCT-based 3D histology using lead(II) acetate.Journal of anatomy · 2021Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-ray microtomography (microCT) enables histological-scale 3D imaging of many types of biological samples, but it has yet to rival traditional histology for differentiation of tissue types and cell components. This report presents prima facie results indicating that a simple lead(II) acetate staining solution can impart preferential X-ray contrast to cell nuclei. While not strictly selective for nuclei, the staining reflects local cell-density differences. It can be applied in a single overnight treatment and does not require hematoxylin staining or drying of the sample. The stain is removable with EDTA, and it may enhance early calcifications. A basic protocol is given as a guide for further testing and optimization.
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