ArticleDigestive diseases and sciences2021
A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies.
Article in Digestive diseases and sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers, 1 of them a synthesis that pooled it.
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12 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.
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- T cell receptors for antigen on intraepithelial cytolytic T lymphocytes in celiac disease engage enterocyte HLA-E and HLA-B.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Insightful Investigations into the Applications of Immunohistochemistry to Highlight Intraepithelial Lymphocytes in Formalin-Fixed and Frozen Duodenal Biopsies: Comparability of Results and Cut-Off Revision.Digestive diseases and sciences · 2026Article
- Fit to a "T"-Immunohistochemical Identification of Intra-Epithelial Lymphocytes in Duodenal Biopsies of Patients with Coeliac Disease.Digestive diseases and sciences · 2026Article
- Immunohistochemistry to Highlight Intraepithelial Lymphocytes in Formalin-Fixed and Frozen Duodenal Biopsies: Comparability of Results and Cut-Off Revision.Digestive diseases and sciences · 2025Article
- A new algorithm for coeliac disease based on the 'long forgotten' TCRγδHistopathology · 2025Article
- A feasibility study using quantitative and interpretable histological analyses of celiac disease for automated cell type and tissue area classification.Scientific reports · 2024Article
- Population-based screening for celiac disease reveals that the majority of patients are undiagnosed and improve on a gluten-free diet.Scientific reports · 2022Article
- UV-induced local immunosuppression in the tumour microenvironment of eccrine porocarcinoma and poroma.Scientific reports · 2022Article
- Apolipoprotein A4 Defines the Villus-Crypt Border in Duodenal Specimens for Celiac Disease Morphometry.Frontiers in immunology · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe histopathologic diagnosis of celiac disease (CD) may be challenging when the duodenal biopsies mucosal injury is limited. Intraepithelial T-lymphocytes (IELs) can be useful to characterize the degree of mucosal inflammation. A small fraction of IELs expresses the γδ T-cell receptor (named γδ-IELs), whose density, determined by flow cytometry or frozen section immunohistochemistry (IHC), is a specific marker for CD.
aimTo establish a new IHC assay for γδ-IELs applicable to formalin-fixed paraffin-embedded (FFPE) duodenal biopsies.
methodsWe analyzed γδ-IELs using IHC in 138 duodenal biopsies using a standard IHC staining protocol with a new monoclonal antibody H-41. IELs were quantitated with digital image analysis.
resultsCompared to those in non-celiac controls (n = 51), γδ-IEL density was significantly increased in newly diagnosed celiac disease patients (n = 22, p < 0.0001). In ROC-curve analysis, the cutoff of 6.5 γδ-IELs/100 enterocytes distinguished optimally active CD patients from non-celiac controls (sensitivity 96%, specificity 95%). γδ-IEL density in CD patients on a gluten-free diet (n = 53) were also higher than in controls (p < 0.0001), but lower than those in newly diagnosed CD (p < 0.0001). The diagnostic value of γδ-IELs outperformed that of CD3 + IELs in both patient groups. γδ-IELs were better than CD3 + IELs distinguishing between celiac disease and conditions histologically mimicking celiac disease (n = 12).
conclusionsIntraepithelial γδ T-lymphocytes can be stained and quantitated reliably in FFPE duodenal biopsies. The results showed excellent specificity and sensitivity for celiac disease. The new IHC method of detection of γδ-IELs is a promising addition to the routine histopathologic assessment methodology of celiac disease.
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