ArticleQuantitative imaging in medicine and surgery2020
Real-time three-dimensional histology-like imaging by label-free nonlinear optical microscopy.
Article in Quantitative imaging in medicine and surgery, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Detecting drug-induced nephrotoxicity using simultaneous label-free autofluorescence multiharmonic microscopy.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Short-pulsed AI-augmented third harmonic generation imaging visualizes negative-contrast cell nuclei in fresh mouse liver tissue without optical-induced damage.Biomedical optics express · 2026Article
- Femtosecond label-free imaging: A rapid and reliable alternative for intraoperative pathological assessment in thoracic oncology.JTCVS open · 2026Article
- Deep Learning-Enabled Virtual Multiplexed Immunostaining of Label-Free Tissue for Vascular Invasion Assessment.BME frontiers · 2026Article
- Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy.Journal of visualized experiments : JoVE · 2025Article
- Enhanced multiscale human brain imaging by semi-supervised digital staining and serial sectioning optical coherence tomography.Light, science & applications · 2025Article
- Label-Free Evaluation of Lung and Heart Transplant Biopsies Using Tissue Autofluorescence-Based Virtual Staining.BME frontiers · 2025Article
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Predicting DNA damage response in non-small cell lung cancer organoids via simultaneous label-free autofluorescence multiharmonic microscopy.Redox biology · 2024Article
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- To label or not: the need for validation in label-free imaging.Journal of biomedical optics · 2024Review
- Enhanced Multiscale Human Brain Imaging by Semi-supervised Digital Staining and Serial Sectioning Optical Coherence Tomography.Research square · 2024Article
- Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two-photon excited fluorescence.Biomedical optics express · 2023Article
- Deep learning-enabled realistic virtual histology with ultraviolet photoacoustic remote sensing microscopy.Nature communications · 2023Article
- A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.International journal of molecular sciences · 2023Review
- Label-free complete absorption microscopy using second generation photoacoustic remote sensing.Scientific reports · 2022Article
- Three-dimensional characterizations of two-photon excitation fluorescence images of elastic fibers affected by cutaneous scar duration.Quantitative imaging in medicine and surgery · 2021Article
- Application of Multiphoton Microscopic Imaging in Study of Gastric Cancer.Technology in cancer research & treatmentReview
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe current gold-standard formalin-fixed and paraffin-embedded (FFPE) histology typically requires several days for tissue fixing, embedding, sectioning, and staining to provide depth-resolved tissue feature visualization. During these time- and labor- intense processes, the
methodsA simultaneous label-free autofluorescence multiharmonic (SLAM) microscope is used to conduct
resultsVarious histological correlations are identified with high similarities for the color-converted histology-like SLAM microscopy images. By applying the color transforming algorithm on real-time SLAM image sequences and 3D SLAM image stacks, we report, for the first time and to the best our knowledge, real-time 3D histology-like imaging. Furthermore, the quantified N/C ratio of the cells in the SLAM image are overlaid on the converted histology-like image as a new image contrast.
conclusionsWe demonstrated real-time 3D histology-like imaging and its future potential using SLAM microscopy aided by color remapping and deep-learning-based feature segmentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.