ArticleNature nanotechnology2021
A DNA-nanoassembly-based approach to map membrane protein nanoenvironments.
Article in Nature nanotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Research Progress and Applications of Multivalent, Multispecific and Modified Nanobodies for Disease Treatment.Frontiers in immunology · 2021Pooled it
- Targeted AAV gene therapy for neuroblastoma via direct capsid-antibody coupling.EMBO molecular medicine · 2026Article
- Interactions of the DNA Nanostructure with Silane-Based Self-Assembled Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Production of Antibody Fragments Suitable for 1:1, Site-Specific Labeling.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Proximity-activated DNA scanning encoded sequencing for massive access to membrane proteins nanoscale organization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Method for Mapping the Membrane Protein Nanoenvironments Using DNA Nanostructures (NanoDeep).Methods in molecular biology (Clifton, N.J.) · 2025Article
- Multiparametric profiling of HER2-enriched extracellular vesicles in breast cancer using Single Extracellular VEsicle Nanoscopy.Journal of nanobiotechnology · 2024Article
- Bidirectional linkage of DNA barcodes for the multiplexed mapping of higher-order protein interactions in cells.Nature biomedical engineering · 2024Article
- Molecular robotic agents that survey molecular landscapes for information retrieval.Nature communications · 2024Article
- CytoDirect: A Nucleic Acid Nanodevice for Specific and Efficient Delivery of Functional Payloads to the Cytoplasm.Journal of the American Chemical Society · 2023Article
- Biological Applications of Synthetic Binders Isolated from a Conceptually New Adhiron Library.Biomolecules · 2023Article
- Macroporous Epoxy-Based Monoliths Functionalized with Anti-CD63 Nanobodies for Effective Isolation of Extracellular Vesicles in Urine.International journal of molecular sciences · 2023Article
- DNA-based customized functional modules for signal transformation.Frontiers in chemistry · 2023Review
- Water-Air Interface to Mimic In Vitro Tumoral Cell Migration in Complex Micro-Environments.Biosensors · 2022Article
- Patterned Carboxymethyl-Dextran Functionalized Surfaces Using Organic Mixed Monolayers for Biosensing Applications.ACS applied bio materials · 2022Article
- Applications of DNA-Functionalized Proteins.International journal of molecular sciences · 2021Review
- Obtaining Precise Molecular Information via DNA Nanotechnology.Membranes · 2021Review
- Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications.Nature communications · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most proteins at the plasma membrane are not uniformly distributed but localize to dynamic domains of nanoscale dimensions. To investigate their functional relevance, there is a need for methods that enable comprehensive analysis of the compositions and spatial organizations of membrane protein nanodomains in cell populations. Here we describe the development of a non-microscopy-based method for ensemble analysis of membrane protein nanodomains. The method, termed nanoscale deciphering of membrane protein nanodomains (NanoDeep), is based on the use of DNA nanoassemblies to translate membrane protein organization information into a DNA sequencing readout. Using NanoDeep, we characterized the nanoenvironments of Her2, a membrane receptor of critical relevance in cancer. Importantly, we were able to modulate by design the inventory of proteins analysed by NanoDeep. NanoDeep has the potential to provide new insights into the roles of the composition and spatial organization of protein nanoenvironments in the regulation of membrane protein function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.