ArticleNature methods2020
BAcTrace, a tool for retrograde tracing of neuronal circuits in Drosophila.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Keeping up with the neighbours: local synchronisation of cell fate decisions during development.EMBO reports · 2026Review
- Synaptic targets of circadian clock neurons influence core clock parameters.Science advances · 2025Article
- Article
- Spatial constraints and cell surface molecule depletion structure a randomly connected learning circuit.Current biology : CB · 2025Article
- Generation of Odorant Receptor-QF2 Knock-In Drivers for Improved Analysis of Olfactory Circuits in Drosophila.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- The lives of cells, recorded.Nature reviews. Genetics · 2025Review
- Single-cell type analysis of wing premotor circuits in the ventral nerve cord ofbioRxiv : the preprint server for biology · 2025Article
- Synaptic Targets of Circadian Clock Neurons Influence Core Clock Parameters.bioRxiv : the preprint server for biology · 2025Article
- Impact of developmental temperature on neural growth, connectivity, and function.Science advances · 2025Article
- A neurotrophin functioning with a Toll regulates structural plasticity in a dopaminergic circuit.eLife · 2024Article
- Advances in the labelling and selective manipulation of synapses.Nature reviews. Neuroscience · 2024Review
- Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Functional labeling of individualized postsynaptic neurons using optogenetics and trans-Tango in Drosophila (FLIPSOT).PLoS genetics · 2024Article
- Enabling neighbour labelling: using synthetic biology to explore how cells influence their neighbours.Development (Cambridge, England) · 2024Review
- Article
- The synaptic basis for sexual dimorphism in the invertebrate nervous system.Current opinion in neurobiology · 2023Review
- Article
- Article
- Genetic dissection of intercellular interactions in vivo by membrane-permeable protein.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Animal behavior is encoded in neuronal circuits in the brain. To elucidate the function of these circuits, it is necessary to identify, record from and manipulate networks of connected neurons. Here we present BAcTrace (Botulinum-Activated Tracer), a genetically encoded, retrograde, transsynaptic labeling system. BAcTrace is based on Clostridium botulinum neurotoxin A, Botox, which we engineered to travel retrogradely between neurons to activate an otherwise silent transcription factor. We validated BAcTrace at three neuronal connections in the Drosophila olfactory system. We show that BAcTrace-mediated labeling allows electrophysiological recording of connected neurons. Finally, in a challenging circuit with highly divergent connections, BAcTrace correctly identified 12 of 16 connections that were previously observed by electron microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.