ArticleScientific reports2020
Assessment of nanoindentation in stiffness measurement of soft biomaterials: kidney, liver, spleen and uterus.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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17 citing papers in PubMed, 37 citations in OpenAlex.
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- Impact of paraben on uterine collagen: An integrated and targeted Correlative approach using second harmonic generation microscopy, nanoindentation, and atomic force microscopy.Journal of the mechanical behavior of biomedical materials · 2025Article
- Programmable phase transition enables tunable microstructures and micromechanics in thermoresponsive polysaccharide hydrogels.Communications materials · 2025Article
- Decellularized Biohybrid Nerve Promotes Motor Axon Projections.Advanced healthcare materials · 2024Article
- Effect of extracellular matrix stiffness on efficacy of Dapagliflozin for diabetic cardiomyopathy.Cardiovascular diabetology · 2024Article
- Use of Nanoindentation in Determination of Regional Biomechanical Properties of Rabbit Cornea After UVA Cross-Linking.Investigative ophthalmology & visual science · 2023Article
- Vaginal Fibroblast Behavior as a Function of Stiffness Changes in a Polyisocyanide Hydrogel for Prolapse Repair.ACS applied bio materials · 2023Article
- AI-dente: an open machine learning based tool to interpret nano-indentation data of soft tissues and materials.Soft matter · 2023Article
- Comparative mucomic analysis of three functionally distinct Cornu aspersum Secretions.Nature communications · 2023Article
- Biological role of matrix stiffness in tumor growth and treatment.Journal of translational medicine · 2022Review
- Corneal Adhesion Possesses the Characteristics of Solid and Membrane.Bioengineering (Basel, Switzerland) · 2022Article
- Polydatin attenuates tubulointerstitial fibrosis in diabetic kidney disease by inhibiting YAP expression and nuclear translocation.Frontiers in physiology · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanoindentation technology with high spatial resolution and force sensitivity is widely used to measure the mechanical properties of hard biomaterials and tissues. However, its reliability to analyze soft biomaterials and organs has not been tested. Here, we evaluated the utility of nanoindentation to measure the passive mechanical properties of soft biological specimen. Kidney, liver, spleen and uterus samples were harvested from C57BL/6 N mice. We assessed test-retest repeatability in biological specimen and hydrogel controls using Bland-Altman diagrams, intraclass correlation coefficients (ICCs) and the within-subject coefficients of variation (COVs). The results were calculated using Hertzian, JKR and Oliver & Pharr models. Similar to hydrogels, Bland-Altman plots of all biological specimen showed good reliability in stiffness test and retest examinations. In gels, ICCs were larger than 0.8 and COVs were smaller than 15% in all three models. In kidney, liver, spleen and uterus, ICCs were consistently larger than 0.8 only in the Hertzian model but not in the JKR and Oliver & Pharr models. Similarly, COVs were consistently smaller than 15% in kidney, liver, spleen and uterus only in the Hertzian model but not in the other models. We conclude that nanoindentation technology is feasible in detecting the stiffness of kidney, liver, spleen and uterus. The Hertzian model is the preferred method to provide reliable results on ex vivo organ stiffness of the biological specimen under study.
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