Evidence map›Paper›PMID 33139727›Full record

ArticleScientific reports2020

Cleavable hairpin beacon-enhanced fluorescence detection of nucleic acid isothermal amplification and smartphone-based readout.

Xiong Ding, Kun Yin, Ziyue Li, Vikram Pandian, Joan A Smyth, Zeinab Helal, Changchun Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiong DingDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA.
Kun YinDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA.
Ziyue LiDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA.
Vikram PandianElectrical and Computer Engineering, University of Texas at Austin, Austin, TX, 78712, USA.
Joan A SmythConnecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT, 06269, USA.
Zeinab HelalConnecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT, 06269, USA.
Changchun LiuDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA. chaliu@uchc.edu.

Funding

Asymmetric CRISPR Approach for Nucleic Acid QuantificationR01EB023607 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI Changchun Liu · 2017 to 2026
$2.4M
Point of Care Diagnostics of HPV-Associated Cervical Cancer in HIV Epidemic Areas in ChinaR01CA214072 · NCI · UNIVERSITY OF PENNSYLVANIA · PI LIU, CHANGCHUN · 2017 to 2021
$771k
Smart Cup: A Mobile Molecular Detection Device for Rapid Diagnosis of Hand Foot and Mouth Disease in ChinaR21TW010625 · FIC · UNIVERSITY OF PENNSYLVANIA · PI LIU, CHANGCHUN · 2017 to 2018
$403k
FIC NIH HHS R21 TW010625FIC NIH HHS R21TW010625NCI NIH HHS R01 CA214072NCI NIH HHS R01CA214072NIBIB NIH HHS R01 EB023607NIBIB NIH HHS R01EB023607
6 · The paper itself

Abstract

Fluorescence detection of nucleic acid isothermal amplification utilizing energy-transfer-tagged oligonucleotide probes provides a highly sensitive and specific method for pathogen detection. However, currently available probes suffer from relatively weak fluorescence signals and are not suitable for simple, affordable smartphone-based detection at the point of care. Here, we present a cleavable hairpin beacon (CHB)-enhanced fluorescence detection for isothermal amplification assay. The CHB probe is a single fluorophore-tagged hairpin oligonucleotide with five continuous ribonucleotides which can be cleaved by the ribonuclease to specifically initiate DNA amplification and generate strong fluorescence signals. By coupling with loop-mediated isothermal amplification (LAMP), the CHB probe could detect Borrelia burgdorferi (B. burgdorferi) recA gene with a sensitivity of 100 copies within 25 min and generated stronger specific fluorescence signals which were easily read and analysed by our programmed smartphone. Also, this CHB-enhanced LAMP (CHB-LAMP) assay was successfully demonstrated to detect B. burgdorferi DNA extracted from tick species, showing comparable results to real-time PCR assay. In addition, our CHB probe was compatible with other isothermal amplifications, such as isothermal multiple-self-matching-initiated amplification (IMSA). Therefore, CHB-enhanced fluorescence detection is anticipated to facilitate the development of simple, sensitive smartphone-based point-of-care pathogen diagnostics in resource-limited settings.

Indexed as

FluorescencePoint-of-Care TestingSmartphoneAnimalsBorrelia burgdorferiDNA, BacterialHumansLyme DiseaseMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesOligonucleotidesRec A RecombinasesRibonucleasesRibonucleotidesSensitivity and SpecificityTicksDNA, BacterialOligonucleotidesRec A RecombinasesRibonucleasesRibonucleotides

Identifiers

PMID33139727
PMCPMC7608614

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.