ArticleNature communications2020
Extensive 5'-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Mechanisms of transcription termination across the coding and noncoding loci of the genome.Nature reviews. Molecular cell biology · 2026Review
- Post-loading ribonucleic acid into lipid nanoparticle carriers.bioRxiv : the preprint server for biology · 2026Article
- RNA regulation in plants.Science China. Life sciences · 2026Review
- The Pof1 nicotinamide mononucleotide adenylyl transferase has a non-canonical role in NADThe Journal of biological chemistry · 2026Article
- Quantification and transcriptome profiling reveal abundant, dynamic and translatable dephospho-CoA-capped RNAs.Nature biotechnology · 2026Article
- NAD+ capping of sibD transcripts in E. coli is mediated by its minimal promoter and enhanced by ppGpp.Nucleic acids research · 2026Article
- The Mysterious World of Non-Canonical Caps - What We Know and Why We Need New Sequencing Techniques.Chembiochem : a European journal of chemical biology · 2025Review
- Identification and in vitro characterization of UDP-GlcNAc-RNA cap-modifying and decapping enzymes.Nucleic acids research · 2024Article
- Hidden features of NAD-RNA epitranscriptome iniScience · 2024Article
- If the 5' cap fits (wear it) - Non-canonical RNA capping.RNA biology · 2024Review
- Identification of NAD-RNA species and ADPR-RNA decapping in Archaea.Nature communications · 2023Article
- Future Perspectives for the Identification and Sequencing of Nicotinamide Adenine Dinucleotide-Capped RNAs.Accounts of chemical research · 2023Article
- Article
- DpCoA tagSeq: Barcoding dpCoA-Capped RNA for Direct Nanopore Sequencing via Maleimide-Thiol Reaction.Analytical chemistry · 2023Article
- Biological Catalysis and Information Storage Have Relied onBiomolecules · 2023Review
- NADcapPro and circNC: methods for accurate profiling of NAD and non-canonical RNA caps in eukaryotes.Communications biology · 2023Article
- Article
- Yeast Dxo1 is required for 25S rRNA maturation and acts as a transcriptome-wide distributive exonuclease.RNA (New York, N.Y.) · 2022Article
- Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA.Nature communications · 2022Article
- Use of NAD tagSeq II to identify growth phase-dependent alterations inProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5'-ends. The modification percentage of transcripts is low (<5%). NAD incorporation occurs mainly during transcription initiation by RNA polymerase II, which uses distinct promoters with a YAAG core motif for this purpose. Most NAD-RNAs are 3'-truncated. At least three decapping enzymes, Rai1, Dxo1, and Npy1, guard against NAD-RNA at different cellular locations, targeting overlapping transcript populations. NAD-mRNAs are not translatable in vitro. Our work indicates that in budding yeast, most of the NAD incorporation into RNA seems to be disadvantageous to the cell, which has evolved a diverse surveillance machinery to prematurely terminate, decap and reject NAD-RNAs.
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