ArticleMicrobial cell factories2020
Intelligent microbial cell factory with genetic pH shooting (GPS) for cell self-responsive base/acid regulation.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Engineering an Optogenetic pH-Modulator in Bacteria.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Engineering the Gut Microbiome: Emerging Genome-Editing Strategies and Therapeutic Applications.Microorganisms · 2026Review
- Temperature-responsive regulation of the polycyclic aromatic hydrocarbon-degrading mesophilic bacteriumApplied and environmental microbiology · 2025Article
- Advances in stress-tolerance elements for microbial cell factories.Synthetic and systems biotechnology · 2024Review
- A synthetic microbial Daisyworld: planetary regulation in the test tube.Journal of the Royal Society, Interface · 2024Article
- Using design of experiments to guide genetic optimization of engineered metabolic pathways.Journal of industrial microbiology & biotechnology · 2024Review
- Practical genetic control strategies for industrial bioprocesses.Journal of industrial microbiology & biotechnology · 2022Review
- Separation and purification of plant terpenoids from biotransformation.Engineering in life sciences · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundIn industrial fermentation, pH fluctuation resulted from microbial metabolism influences the strain performance and the final production. The common way to control pH is adding acid or alkali after probe detection, which is not a fine-tuned method and often leads to increased costs and complex downstream processing. Here, we constructed an intelligent pH-sensing and controlling genetic circuits called "Genetic pH Shooting (GPS)" to realize microbial self-regulation of pH.
resultsIn order to achieve the self-regulation of pH, GPS circuits consisting of pH-sensing promoters and acid-/alkali-producing genes were designed and constructed. Designed pH-sensing promoters in the GPS can respond to high or low pHs and generate acidic or alkaline substances, achieving endogenously self-responsive pH adjustments. Base shooting circuit (BSC) and acid shooting circuit (ASC) were constructed and enabled better cell growth under alkaline or acidic conditions, respectively. Furthermore, the genetic circuits including GPS, BSC and ASC were applied to lycopene production with a higher yield without an artificial pH regulation compared with the control under pH values ranging from 5.0 to 9.0. In scale-up fermentations, the lycopene titer in the engineered strain harboring GPS was increased by 137.3% and ammonia usage decreased by 35.6%.
conclusionsThe pH self-regulation achieved through the GPS circuits is helpful to construct intelligent microbial cell factories and reduce the production costs, which would be much useful in industrial applications.
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