Evidence map›Paper›PMID 33138172›Full record

ArticlePharmaceutics2020

A Versatile Macromer-Based Glycosaminoglycan (sHA3) Decorated Biomaterial for Pro-Osteogenic Scavenging of Wnt Antagonists.

Mathis Gronbach, Franziska Mitrach, Stephanie Möller, Sandra Rother, Sabrina Friebe, Stefan G Mayr, Matthias Schnabelrauch, Vera Hintze, Michael C Hacker, Michaela Schulz-Siegmund

Abstract read
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In one paragraph

Article in Pharmaceutics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mathis GronbachPharmaceutical Technology, Medical Faculty, University of Leipzig, Eilenburger Str. 15A, 04317 Leipzig, Germany.
Franziska MitrachPharmaceutical Technology, Medical Faculty, University of Leipzig, Eilenburger Str. 15A, 04317 Leipzig, Germany.
Stephanie MöllerBiomaterials Department, INNOVENT e.V., Pruessingstraße 27B, 07745 Jena, Germany.
Sandra RotherMax Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str. 27, 01062 Dresden, Germany.
Sabrina FriebeLeibniz-Institut für Oberflächenmodifizierung e.V. (IOM), Permoserstr. 15, 04318 Leipzig, Germany.
Stefan G MayrLeibniz-Institut für Oberflächenmodifizierung e.V. (IOM), Permoserstr. 15, 04318 Leipzig, Germany.
Matthias SchnabelrauchBiomaterials Department, INNOVENT e.V., Pruessingstraße 27B, 07745 Jena, Germany.ORCID 0000-0002-1968-8058
Vera HintzeMax Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str. 27, 01062 Dresden, Germany.
Michael C HackerPharmaceutical Technology, Medical Faculty, University of Leipzig, Eilenburger Str. 15A, 04317 Leipzig, Germany.
Michaela Schulz-SiegmundPharmaceutical Technology, Medical Faculty, University of Leipzig, Eilenburger Str. 15A, 04317 Leipzig, Germany.

Funding

Deutsche Forschungsgemeinschaft 420160411Deutsche Forschungsgemeinschaft 59307082
6 · The paper itself

Abstract

High serum levels of Wnt antagonists are known to be involved in delayed bone defect healing. Pharmaceutically active implant materials that can modulate the micromilieu of bone defects with regard to Wnt antagonists are therefore considered promising to support defect regeneration. In this study, we show the versatility of a macromer based biomaterial platform to systematically optimize covalent surface decoration with high-sulfated glycosaminoglycans (sHA3) for efficient scavenging of Wnt antagonist sclerostin. Film surfaces representing scaffold implants were cross-copolymerized from three-armed biodegradable macromers and glycidylmethacrylate and covalently decorated with various polyetheramine linkers. The impact of linker properties (size, branching) and density on sHA3 functionalization efficiency and scavenging capacities for sclerostin was tested. The copolymerized 2D system allowed for finding an optimal, cytocompatible formulation for sHA3 functionalization. On these optimized sHA3 decorated films, we showed efficient scavenging of Wnt antagonists DKK1 and sclerostin, whereas Wnt agonist Wnt3a remained in the medium of differentiating SaOS-2 and hMSC. Consequently, qualitative and quantitative analysis of hydroxyapatite staining as a measure for osteogenic differentiation revealed superior mineralization on sHA3 materials. In conclusion, we showed how our versatile material platform enables us to efficiently scavenge and inactivate Wnt antagonists from the osteogenic micromilieu. We consider this a promising approach to reduce the negative effects of Wnt antagonists in regeneration of bone defects via sHA3 decorated macromer based macroporous implants.

Indexed as

biodegradable polymerbone regenerationDKK1hyaluronanscavengingsclerostinsurface modificationtissue engineeringWnt-signaling

Identifiers

PMID33138172
PMCPMC7693161

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.