Evidence map›Paper›PMID 33138102›Full record

ReviewMembranes2020

Revealing Plasma Membrane Nano-Domains with Diffusion Analysis Methods.

Jakob L Kure, Camilla B Andersen, Kim I Mortensen, Paul W Wiseman, Eva C Arnspang

Abstract readReview
In one paragraph

Review in Membranes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Polar Glycerolipids and Membrane Lipid Rafts.International journal of molecular sciences · 2024
    Review
  4. Review
  5. Article
  6. [Simulation of gas chromatographic separation based on random diffusion].Se pu = Chinese journal of chromatography · 2022
    Article
  7. Article
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jakob L KureSDU Biotechnology, Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.ORCID 0000-0003-1959-2811
Camilla B AndersenSDU Biotechnology, Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.ORCID 0000-0003-1298-0598
Kim I MortensenDTU Health Tech, Department of Health Technology, Technical University of Denmark, Ørsteds Plads, Building 345C, 2800 Kgs. Lyngby, Denmark.ORCID 0000-0002-2903-8999
Paul W WisemanDepartment of Chemistry, McGill University, 801 Sherbrooke St. West, Montréal, QC H3A 0B8, Canada.ORCID 0000-0002-5732-2858
Eva C ArnspangSDU Biotechnology, Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.

Funding

Novo Nordisk Fonden NNF16OC0022166Villum Fonden 19105
6 · The paper itself

Abstract

Nano-domains are sub-light-diffraction-sized heterogeneous areas in the plasma membrane of cells, which are involved in cell signalling and membrane trafficking. Throughout the last thirty years, these nano-domains have been researched extensively and have been the subject of multiple theories and models: the lipid raft theory, the fence model, and the protein oligomerization theory. Strong evidence exists for all of these, and consequently they were combined into a hierarchal model. Measurements of protein and lipid diffusion coefficients and patterns have been instrumental in plasma membrane research and by extension in nano-domain research. This has led to the development of multiple methodologies that can measure diffusion and confinement parameters including single particle tracking, fluorescence correlation spectroscopy, image correlation spectroscopy and fluorescence recovery after photobleaching. Here we review the performance and strengths of these methods in the context of their use in identification and characterization of plasma membrane nano-domains.

Indexed as

diffusionfluorescence correlation spectroscopyimage correlation spectroscopylipid raftnano-domainsingle-particle tracking

Identifiers

PMID33138102
PMCPMC7693849

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.