ArticlePLoS genetics2020
NIGT1 family proteins exhibit dual mode DNA recognition to regulate nutrient response-associated genes in Arabidopsis.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- HHO5 orchestrates dose-dependent feedback regulation of organic versus inorganic nitrogen signaling in Arabidopsis.The Plant cell · 2026Article
- Progress and new questions in understanding the mechanisms controlling plant nitrogen responses.Plant biotechnology (Tokyo, Japan) · 2026Article
- Model-to-crop conserved NUE Regulons enhance machine learning predictions of nitrogen use efficiency.The Plant cell · 2025Article
- Two TAL effectors of Xanthomonas citri promote pustule formation by directly repressing the expression of GRAS transcription factor in citrus.Molecular horticulture · 2025Article
- Genome-wide identification and analysis of phosphate utilization related genes (PURs) reveal their roles involved in low phosphate responses in Brassica napus L.BMC plant biology · 2025Article
- TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat.Nature communications · 2025Article
- Down-regulation of the rice HRS1 HOMOLOG3 transcriptional repressor gene due to N deficiency directly co-activates ammonium and phosphate transporter genes.Journal of experimental botany · 2025Article
- Rice transcription factorsFrontiers in plant science · 2025Article
- StCDF1: A 'jack of all trades' clock output with a central role in regulating potato nitrate reduction activity.The New phytologist · 2025Article
- HASTY-mediated miRNA dynamics modulate nitrogen starvation-induced leaf senescence in Arabidopsis.Nature communications · 2024Article
- Update of phosphate transport regulations.Journal of plant research · 2024Article
- Role of GARP family transcription factors in the regulatory network for nitrogen and phosphorus acquisition.Journal of plant research · 2024Review
- Transcription factor module NLP-NIGT1 fine-tunes NITRATE TRANSPORTER2.1 expression.Plant physiology · 2023Article
- Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice.Frontiers in plant science · 2023Article
- The apple GARP family gene MdHHO3 regulates the nitrate response and leaf senescence.Frontiers in plant science · 2022Article
- Genome-Wide Identification and Characterization ofFrontiers in plant science · 2022Article
- The Dynamics of NO3- and NH4+ Uptake in Duckweed Are Coordinated with the Expression of Major Nitrogen Assimilation Genes.Plants (Basel, Switzerland) · 2021Article
- Adjusting plant nutrient acquisition to fluctuating availability: transcriptional co-regulation of the nitrate and phosphate deprivation responses in roots.Journal of experimental botany · 2021Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Fine-tuning of nutrient uptake and response is indispensable for maintenance of nutrient homeostasis in plants, but the details of underlying mechanisms remain to be elucidated. NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) family proteins are plant-specific transcriptional repressors that function as an important hub in the nutrient signaling network associated with the acquisition and use of nitrogen and phosphorus. Here, by yeast two-hybrid assays, bimolecular fluorescence complementation assays, and biochemical analysis with recombinant proteins, we show that Arabidopsis NIGT1 family proteins form a dimer via the interaction mediated by a coiled-coil domain (CCD) in their N-terminal regions. Electrophoretic mobility shift assays defined that the NIGT1 dimer binds to two different motifs, 5'-GAATATTC-3' and 5'-GATTC-N38-GAATC-3', in target gene promoters. Unlike the dimer of wild-type NIGT1 family proteins, a mutant variant that could not dimerize due to amino acid substitutions within the CCD had lower specificity and affinity to DNA, thereby losing the ability to precisely regulate the expression of target genes. Thus, expressing the wild-type and mutant NIGT1 proteins in the nigt1 quadruple mutant differently modified NIGT1-regulated gene expression and responses towards nitrate and phosphate. These results suggest that the CCD-mediated dimerization confers dual mode DNA recognition to NIGT1 family proteins, which is necessary to make proper controls of their target genes and nutrient responses. Intriguingly, two 5'-GATTC-3' sequences are present in face-to-face orientation within the 5'-GATTC-N38-GAATC-3' sequence or its complementary one, while two 5'-ATTC-3' sequences are present in back-to-back orientation within the 5'-GAATATTC-3' or its complementary one. This finding suggests a unique mode of DNA binding by NIGT1 family proteins and may provide a hint as to why target sequences for some transcription factors cannot be clearly determined.
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