ArticleJournal of visualized experiments : JoVE2020
Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells.Current biology : CB · 2024Article
- An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells.bioRxiv : the preprint server for biology · 2024Article
- Live Imaging and Analysis of Meiotic Cytokinesis inBio-protocol · 2024Article
- Visceral mesoderm signaling regulates assembly position and function of the Drosophila testis niche.Developmental cell · 2022Article
- Time-lapse imaging ofSTAR protocols · 2022Article
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Authors and funding
4 authors.
Funding
Abstract
The Drosophila melanogaster male embryonic gonad is an advantageous model to study various aspects of developmental biology including, but not limited to, germ cell development, piRNA biology, and niche formation. Here, we present a dissection technique to live-image the gonad ex vivo during a period when in vivo live-imaging is highly ineffective. This protocol outlines how to transfer embryos to an imaging dish, choose appropriately-staged male embryos, and dissect the gonad from its surrounding tissue while still maintaining its structural integrity. Following dissection, gonads can be imaged using a confocal microscope to visualize dynamic cellular processes. The dissection procedure requires precise timing and dexterity, but we provide insight on how to prevent common mistakes and how to overcome these challenges. To our knowledge this is the first dissection protocol for the Drosophila embryonic gonad, and will permit live-imaging during an otherwise inaccessible window of time. This technique can be combined with pharmacological or cell-type specific transgenic manipulations to study any dynamic processes occurring within or between the cells in their natural gonadal environment.
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Registered trials
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