ArticleResearch and practice in thrombosis and haemostasis2020
Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish.
Article in Research and practice in thrombosis and haemostasis, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- Piggyback knockdown screening of unique genes of zebrafish young thrombocytes identifies eight novel genes in thrombopoiesis.Scientific reports · 2025Article
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- Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish.Research and practice in thrombosis and haemostasis · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBlood clotting in humans is initiated by the binding of tissue factor to activated coagulation factor VII (FVIIa) in the plasma. Previous studies have reported that hepsin and factor VII (FVII)-activating protease are responsible for generating FVIIa.
objectivesWe aimed to identify other proteases that may activate FVII using zebrafish as a model.
methodsWe screened 179 genes encoding serine protease domains using the piggyback knockdown method to identify genes involved in the activation of zebrafish Fvii. A prolonged kinetic prothrombin time (kPT) assay was used to detect gene knockdown effects.
resultsIn the primary screen, 21 genes showed prolonged kPT. In the secondary screen, 14 of 21 genes showed positive results. In the tertiary screen, all 14 genes showed prolonged kPT. These 14 genes were knocked down again to estimate relative levels of zebrafish Fviia. Six genes, including known genes, such as
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