Evidence map›Paper›PMID 33134665›Full record

ArticleACS omega2020

Terbium Excitation Spectroscopy as a Detection Method for Chromatographic Separation of Lanthanide-Binding Biomolecules.

Wojciech Jurkowski, Marcus Heilmann, Anna M Becker, Rainer Buchholz, Thomas B Brück

Open access · goldAbstract read
In one paragraph

Article in ACS omega, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Isolation and Investigation of Natural Rare Earth Metal Chelating Agents FromFrontiers in bioengineering and biotechnology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Wojciech JurkowskiWerner Siemens Chair of Synthetic Biotechnology, Technical University of Munich (TUM), Lichtenbergstr. 4, D-85748 Garching, Germany.
Marcus HeilmannMolecular Imaging and Radiochemistry, Clinic of Nuclear Medicine, Friedrich-Alexander Universität Erlangen-Nürnberg, Paul-Gordan-Str 3, D-91052 Erlangen, Germany.
Anna M BeckerInstitute of Bioprocess Engineering, Friedrich-Alexander Universität Erlangen-Nürnberg, Paul-Gordan-Str 3, D-91052 Erlangen, Germany.
Rainer BuchholzInstitute of Bioprocess Engineering, Friedrich-Alexander Universität Erlangen-Nürnberg, Paul-Gordan-Str 3, D-91052 Erlangen, Germany.
Thomas B BrückWerner Siemens Chair of Synthetic Biotechnology, Technical University of Munich (TUM), Lichtenbergstr. 4, D-85748 Garching, Germany.
Friedrich-Alexander-Universität Erlangen-Nürnberg · DESiemens (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studies of biosorption and bioaccumulation of heavy metals deal mostly with challenging, inhomogeneous, and complex materials. Therefore, most reports describe only application studies, while fundamental research is limited to indirect methods and speculations on the binding mechanisms. In this study, we describe a method for detecting and isolating heavy metal-binding biomolecules directly from crude extracts. The underlying principle is terbium sensitization and fluorescence excitation spectroscopy used offline after a chromatographic run. Compounds interacting with metal ions inevitably change the coordination sphere of terbium, which is reflected in the excitation spectrum leading to metal-specific luminescence. Main advantages of our approach include simple, fast, and inexpensive experiment design, nondestructive measurements, and detection limits far below 1 mg. Here, we have applied our method for three promising biosorbents (green algae, moss, and cyanobacterium) and obtained first information on the character of active compounds isolated from each species.

Identifiers

PMID33134665
PMCPMC7593993
OpenAlexW3095699490

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.