Evidence map›Paper›PMID 33133688›Full record

ArticleJournal of advanced research2020

Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis

Pengcheng Li, Junhui Xing, Jielei Zhang, Jianwu Jiang, Xuemeng Liu, Di Zhao, Yanzhou Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 47 citations in OpenAlex.

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  16. Role of LncRNAs in the Pathogenesis of Coronary Artery Disease.Reviews in cardiovascular medicine · 2023
    Review
  17. Article
  18. Epigenetic Regulation of Angiogenesis in Peripheral Artery Disease.Methodist DeBakey cardiovascular journal · 2023
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Pengcheng LiDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Junhui XingDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Jielei ZhangDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Jianwu JiangDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Xuemeng LiuDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Di ZhaoDivision of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Yanzhou ZhangDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
First Affiliated Hospital of Zhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionIn atherosclerotic lesions, extensive inflammation of the vessel wall contributes to plaque instability. Long noncoding RNAs (lncRNAs) play important roles in diverse biological processes in atherosclerosis.

objectivesHere, we aim to identify the functional role and regulatory mechanisms of lncRNA hypoxia-inducible factor 1 alpha-antisense RNA 2 (HIF1A-AS2) in atherosclerotic inflammation.

methodsAn atherosclerotic mouse model was induced in ApoE-/- mice by high fat diet (HFD). Endothelial cells (ECs), human aortic smooth muscle cells (SMCs) or human coronary artery endothelial cells (HCAECs) were exposed to ox-LDL to develop the in vitro model. The effects of lncRNA HIF1A-AS2 on inflammation were evaluated by determining levels of inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) and levels of adhesion molecules vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), and macrophage cationic peptide 1 (MCP-1).

resultsIt was established that lncRNA HIF1A-AS2 and ATF2 were highly expressed in atherosclerotic ApoE-/- mice. Downregulating lncRNA HIF1A-AS2 in ox-LDL-exposed ECs, SMCs and HCAECs inhibited inflammation by reducing levels of pro-inflammatory factors and adhesion molecules. LncRNA HIF1A-AS2 bound to the transcription factor USF1 to elevate ATF2 expression. USF1 overexpression counteracted the suppressive effect of lncRNA HIF1A-AS2 silencing on ox-LDL-induced inflammation. Knockdown of lncRNA HIF1A-AS2 or ATF2 could also attenuate inflammation in atherosclerotic mice. Collectively, the present study demonstrates that downregulation of lncRNA HIF1A-AS2 represses the binding of USF1 to the ATF2 promoter region and then inhibits ATF2 expression, thereby suppressing atherosclerotic inflammation.

conclusionThis study suggests lncRNA HIF1A-AS2 as an promising therapeutic target for atherosclerosis.

Indexed as

Activating transcription factorATCC, American Type Culture CollectionATF2, activating transcription factor 2AtherosclerosisCAD, coronary artery diseaseCCK-8, cell counting kit-8ChIP, Chromatin immunoprecipitationDMEM, Dulbecco’s modified Eagle’s mediumECs, endothelial cellsELISA, enzyme linked immunosorbent assayGAPDH, Glyceraldehyde-3-phosphate dehydrogenaseHCAECs, human coronary artery endothelial cellsHE, Hematoxylin-eosinHFD, high fat dietHIF1A-AS2, hypoxia-inducible factor 1 alpha-antisense RNA 2Hypoxia-inducible factor 1 alpha-antisense RNA 2ICAM-1, intercellular adhesion molecule-1IgG, immunoglobulin GIL-1β, interleukin-1βIL-6, interleukin-6InflammationLDL, low-density lipoproteinlncRNAs, long noncoding RNAsLong noncoding RNAMCP-1, monocyte chemoattractant protein-1ND, normal dietox-LDL, oxidized-low-density lipoproteinPBS, phosphate buffered salineRIP, RNA binding protein immunoprecipitationRT-qPCR, reverse transcription quantitative polymerase chain reactionsh, short hairpin RNAsi-NC, small interfering RNA-negative controlSMCs, smooth muscle cellsTNF-α, tumor necrosis factor-αTranscription factorUpstream transcription factor 1USF1, upstream stimulatory factor 1VCAM-1, vascular cell adhesion molecule 1

Identifiers

PMID33133688
PMCPMC7584671
OpenAlexW3045979090

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.