ArticleJournal of advanced research2020
TRPV4 promotes acoustic wave-mediated BBB opening via Ca
Article in Journal of advanced research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Towards standardization of the parameters for opening the blood-brain barrier with focused ultrasound to treat glioblastoma multiforme: A systematic review of the devices, animal models, and therapeutic compounds used in rodent tumor models.Frontiers in oncology · 2022Pooled it
- Focused ultrasound for cellular mechanoactivation and nanomedicine delivery in cancer.Biomedical microdevices · 2026Review
- The role of TRPV4 in ischemic stroke.Molecular brain · 2026Review
- Advancements in Therapy for Alzheimer's Disease Based on the Cerebral Lymphatic System.Degenerative neurological and neuromuscular disease · 2026Article
- Investigation into the synergistic effect of atorvastatin combined with ultrasound stimulation for anti-glioma therapy.Oncology letters · 2025Article
- Very Low-Intensity Ultrasound Facilitates Glymphatic Influx and Clearance via Modulation of the TRPV4-AQP4 Pathway.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Nanotherapeutics for Alleviating Anesthesia-Associated Complications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Enhanced macromolecular substance extravasation through the blood-brain barrier via acoustic bubble-cell interactions.Ultrasonics sonochemistry · 2024Article
- A multicellular brain spheroid model for studying the mechanisms and bioeffects of ultrasound-enhanced drug penetration beyond the blood‒brain barrier.Scientific reports · 2024Article
- The effect of combined ultrasound stimulation and gastrodin on seizures in mice.Frontiers in neuroscience · 2024Article
- The protective effects of gastrodin on neurological disorders: an update and future perspectives.Frontiers in pharmacology · 2024Review
- Electromagnetic fields regulate calcium-mediated cell fate of stem cells: osteogenesis, chondrogenesis and apoptosis.Stem cell research & therapy · 2023Review
- Characterization of TRPV4-mediated signaling pathways in an optimized human choroid plexus epithelial cell line.American journal of physiology. Cell physiology · 2022Article
- Article
- Facilitating drug delivery in the central nervous system by opening the blood-cerebrospinal fluid barrier with a single low energy shockwave pulse.Fluids and barriers of the CNS · 2022Article
- Noninvasive Ultrasound Retinal Stimulation for Vision Restoration at High Spatiotemporal Resolution.BME frontiers · 2022Article
- FRET Visualization of Cyclic Stretch-Activated ERKFrontiers in cell and developmental biology · 2022Article
- Investigation of the Therapeutic Effect of Doxorubicin Combined With Focused Shockwave on Glioblastoma.Frontiers in oncology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionNumerous studies have shown the ability of low-energy acoustic waves such as focused ultrasound or shockwave to transiently open blood-brain barrier (BBB) and facilitate drug delivery to the brain. Preclinical and clinical evidences have well demonstrated the efficacy and safety in treating various brain disorders. However, the molecular mechanisms of acoustic waves on the BBB are still not fully understood.
objectivesThe present study aimed at exploring the possible molecular mechanisms of acoustic wave stimulation on brains. METHODS BRIEFLY DESCRIBE THE EXPERIMENTAL
designThe left hemisphere of the rat's brain was treated with pulsed ultrasound from a commercial focused shockwave or a planar ultrasound device, and the right hemisphere served as a control. One hour after the mechanical wave stimulation or overnight, the rats were sacrificed and the brains were harvested for protein or histological analysis. Agonists and antagonists related to the signal transduction pathways of tight junction proteins were used to investigate the possible intracellular mechanisms.
resultsIntracellular signal transduction analysis shows calcium influx through transient receptor potential vanilloid 4 (TRPV4) channels, and the activation of PKC-δ pathway to mediate dissociation of ZO-1 and occludin after acoustic wave stimulation. The activation of TRPV4 or PKC-δ signaling further increased the expression level of TRPV4, suggesting a feedback loop to regulate BBB permeability. Moreover, the tight junction proteins dissociation can be reversed by administration of PKC-δ inhibitor and TRPV4 antagonist.
conclusionThe present study shows the crucial role of TRPV4 in acoustic wave-mediated BBB permeability, specifically its effect on compromising tight junction proteins, ZO-1 and occludin. Our findings provide a new molecular perspective to explain acoustic wave-mediated BBB opening. Moreover, activation of TRPV4 by agonists may reduce the threshold intensity level of acoustic waves for BBB opening, which may prevent undesirable mechanical damages while maintaining efficient BBB opening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.