Designing an enzyme-linked immunosorbent assay for detection of anti-penicillin antibodies levels in various species of animals in Khuzestan province, Iran.

The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the reactive antibody value against penicillin in various species of animals. The serum samples were collected from nine species (forty mature animals in each species) including horse, dog, goat, sheep, buffalo, cattle, donkey, chicken, and fish. The concentrations of total antibody and immunoglobulin M (IgM) against penicillin were detected using an in-house ELISA test. The total anti-penicillin antibodies concentration from high to low in animals was as chicken, horse, fish, donkey, dog, goat, sheep, buffalo, and cattle, respectively. In cattle and sheep, the level of anti-penicillin IgM (APM) was significantly higher than non-IgM antibodies (APNM). Moreover, levels of APNM were very low in chicken and fish serums; no difference was seen regarding these values in buffalo and goat. The other species had significantly lower APM than the APNM. The ani-penicillin antibody levels in the noted animals were successfully detected using the developed ELISA. Most of the species have anti-penicillin antibodies; however, they have reactive antibodies with differences in levels and isotypes.