Evidence map›Paper›PMID 33131040›Full record

ArticleEnvironmental science and pollution research international2021

One-step procedure for peroxidase concentration, dye separation, and color removal by aqueous two-phase system.

Natalia Klanovicz, Andressa Warken, Letícia Paliga, Aline Frumi Camargo, Thamarys Scapini, Jaqueline Garda Buffon, Gislaine Fongaro, Antonio Carlos Silva Costa Teixeira, Helen Treichel

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Article in Environmental science and pollution research international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Natalia KlanoviczResearch Group in Advanced Oxidation Processes (AdOx), Department of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo, Brazil.
Andressa WarkenLaboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil.
Letícia PaligaLaboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil.
Aline Frumi CamargoLaboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil.
Thamarys ScapiniLaboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil.
Jaqueline Garda BuffonMycotoxin and Food Science Laboratory, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, Brazil.
Gislaine FongaroLaboratory of Virology, Federal University of Santa Catarina, Florianópolis, Brazil.
Antonio Carlos Silva Costa TeixeiraResearch Group in Advanced Oxidation Processes (AdOx), Department of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo, Brazil.
Helen TreichelLaboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil. helentreichel@gmail.com.ORCID http://orcid.org/0000-0002-3810-3000

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of our study is to develop a one-step procedure to remove and degrade dyes from wastewater using a low-cost and efficient system based on aqueous two-phase system (ATPS), a well-known technique used to concentrate and recover enzymes. We investigated the catalytic proprieties of rice bran peroxidase (RBP) and found that this homemade enzyme can remain bound to its substrate for up to 5 days in controlled environments, without denaturing and while maintaining stable oxidation reduction potential (ORP) and pH. This biomolecule showed affinity for the ATPS technique prepared with polyethylene glycol and salt, which improved the relative activity up to 170%. The red dye separation in ATPS top phase was achieved in 3 min, in the RBP presence, with 100% of efficiency, and color removal of 87% was obtained in 24 h of enzymatic reaction. The process has promise to be scaled up to 10-fold and to reuse the reagents from the bottom phase of the ATPS.

Indexed as

Coloring AgentsPeroxidaseColorHydrogen-Ion ConcentrationPeroxidasesPolyethylene GlycolsWastewaterWaterColoring AgentsPeroxidasePeroxidasesPolyethylene GlycolsWastewaterWaterDye extractionEnzymatic-assisted treatmentHomemade enzymeReagent reuseScale-upWastewater decolorization

Identifiers

PMID33131040

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.