ArticleBMC genomics2020
Identification of diverse cell populations in skeletal muscles and biomarkers for intramuscular fat of chicken by single-cell RNA sequencing.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- Unraveling the spatial landscape of dystrophinopathies: a transcriptomic approach to Becker and Duchenne muscular dystrophies.The Journal of pathology · 2026Article
- Insights into speckled eggs provided by single-cell transcriptomics in high-yield laying hens.Poultry science · 2026Article
- Impacts of genetic selection on satellite cell function in poultry.Frontiers in physiology · 2026Review
- Leptin receptor signaling mediates the distinct tendon-bone interface reconstruction in rotator cuff tears and osteoporosis-comorbid rotator cuff tears.Stem cell research & therapy · 2025Article
- Research advances in intramuscular fat deposition and chicken meat quality: genetics and nutrition.Journal of animal science and biotechnology · 2025Review
- Single-cell RNA transcriptome uncovers distinct developmental trajectories in the embryonic skeletal muscle of Daheng broiler and Tibetan chicken.BMC genomics · 2025Article
- Multi-Omics Insights into Regulatory Mechanisms Underlying Differential Deposition of Intramuscular and Abdominal Fat in Chickens.Biomolecules · 2025Review
- Article
- Innovative Insights into Single-Cell Technologies and Multi-Omics Integration in Livestock and Poultry.International journal of molecular sciences · 2024Review
- A Single-Cell Atlas of Porcine Skeletal Muscle Reveals Mechanisms That Regulate Intramuscular Adipogenesis.International journal of molecular sciences · 2024Article
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
- Single-cell transcriptomic reveals a cell atlas and diversity of chicken amygdala responded to social hierarchy.iScience · 2024Article
- Building Haplotype-Resolved 3D Genome Maps of Chicken Skeletal Muscle.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Cell Heterogeneity Analysis Revealed the Key Role of Fibroblasts in the Magnum Regression of Ducks.Animals : an open access journal from MDPI · 2024Article
- Integrated meta-omics reveals the regulatory landscape involved in lipid metabolism between pig breeds.Microbiome · 2024Article
- Genomic Insights into Molecular Regulation Mechanisms of Intramuscular Fat Deposition in Chicken.Genes · 2023Review
- White Adipose Tissue Heterogeneity in the Single-Cell Era: From Mice and Humans to Cattle.Biology · 2023Review
- Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development.Foods (Basel, Switzerland) · 2023Article
- Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck.BMC genomics · 2022Article
- Assessing Population Structure and Signatures of Selection in Wanbei Pigs Using Whole Genome Resequencing Data.Animals : an open access journal from MDPI · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundThe development of skeletal muscle is closely related to the efficiency of meat production and meat quality. Chicken skeletal muscle development depends on myogenesis and adipogenesis and occurs in two phases-hyperplasia and hypertrophy. However, cell profiles corresponding to the two-phase muscle development have yet to be determined. Single-cell RNA-sequencing (scRNA-seq) can elucidate the cell subpopulations in tissue and capture the gene expression of individual cells, which can provide new insights into the myogenesis and intramuscular adipogenesis.
resultsTen cell clusters at the post-hatching developmental stage at Day 5 and seven cell clusters at the late developmental stage at Day 100 were identified in chicken breast muscles by scRNA-seq. Five myocyte-related clusters and two adipocyte clusters were identified at Day 5, and one myocyte cluster and one adipocyte cluster were identified at Day 100. The pattern of cell clustering varied between the two stages. The cell clusters showed clear boundaries at the terminal differentiation stage at Day 100; by contrast, cell differentiation was not complete at Day 5. APOA1 and COL1A1 were selected from up-regulated genes in the adipocyte cluster and found to be co-expressed with the ADIPOQ adipocyte marker gene in breast muscles by RNA in situ hybridization.
conclusionsThis study is the first to describe the heterogeneity of chicken skeletal muscle at two developmental stages. The genes APOA1 and COL1A1 were identified as biomarkers for chicken intramuscular fat cells.
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