Evidence map›Paper›PMID 33129190›Full record

ArticleBiomaterials2021

Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.

Zheng Zhong, Xiaoqian Deng, Pengrui Wang, Claire Yu, Wisarut Kiratitanaporn, Xiaokang Wu, Jacob Schimelman, Min Tang, Alis Balayan, Emmie Yao and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Review
  6. Lithography-based 3D printing of hydrogels.Nature reviews bioengineering · 2025
    Article
  7. Review
  8. Tissue Engineering and Ophthalmology.Turkish journal of ophthalmology · 2024
    Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Digital Light Processing Bioprinting Advances for Microtissue Models.ACS biomaterials science & engineering · 2022
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Zheng ZhongDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Xiaoqian DengDepartment of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
Pengrui WangMaterials Science and Engineering Program, University of California San Diego, La Jolla, CA, 92093, USA.
Claire YuDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Wisarut KiratitanapornDepartment of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA.
Xiaokang WuSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.
Jacob SchimelmanDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Min TangDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Alis BalayanSchool of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Emmie YaoDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Jing TianDepartment of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA.
Luwen ChenDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
Kang ZhangDepartment of Ophthalmology, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address: kangzhang@gmail.com.
Shaochen ChenDepartment of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California San Diego, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address: chen168@eng.ucsd.edu.
University of California, San Diego · USLa Jolla Bioengineering Institute · USCedars-Sinai Smidt Heart Institute · USUniversity of California, Los Angeles · US

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jesse Nodora · 1985 to 2026
$124.9M
U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Biomechanics of Leukocytes and Molecular Imaging AgentsR01EB002185 · NIBIB · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI LAWRENCE, MICHAEL BRANDEAU · 2003 to 2010
$5.7M
Rapid 3D bioprinting of biomimetic vascularized tissue constructsR01EB021857 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, DOKMECI, MEHMET REMZI · 2016 to 2019
$2.1M
A stem cell based optic nerve model for studies of axon guidance and regenerationR21EY031122 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, WAHLIN, KARL J · 2019 to 2019
$299k
NCI NIH HHS P30 CA023100NEI NIH HHS R21 EY031122NIBIB NIH HHS R01 EB002185NIBIB NIH HHS R01 EB021857NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Ocular surface diseases including conjunctival disorders are multifactorial progressive conditions that can severely affect vision and quality of life. In recent years, stem cell therapies based on conjunctival stem cells (CjSCs) have become a potential solution for treating ocular surface diseases. However, neither an efficient culture of CjSCs nor the development of a minimally invasive ocular surface CjSC transplantation therapy has been reported. Here, we developed a robust in vitro expansion method for primary rabbit-derived CjSCs and applied digital light processing (DLP)-based bioprinting to produce CjSC-loaded hydrogel micro-constructs for injectable delivery. Expansion medium containing small molecule cocktail generated fast dividing and highly homogenous CjSCs for more than 10 passages in feeder-free culture. Bioprinted hydrogel micro-constructs with tunable mechanical properties enabled the 3D culture of CjSCs while supporting viability, stem cell phenotype, and differentiation potency into conjunctival goblet cells. These hydrogel micro-constructs were well-suited for scalable dynamic suspension culture of CjSCs and were successfully delivered to the bulbar conjunctival epithelium via minimally invasive subconjunctival injection. This work integrates novel cell culture strategies with bioprinting to develop a clinically relevant injectable-delivery approach for CjSCs towards the stem cell therapies for the treatment of ocular surface diseases.

Indexed as

BioprintingAnimalsCell DifferentiationHydrogelsPrinting, Three-DimensionalQuality of LifeRabbitsStem CellsTissue EngineeringHydrogels3D cultureBioprintingConjunctival stem cellInjectable deliveryOcular surface regenerationStem cell therapy

Identifiers

PMID33129190
PMCPMC7719077
OpenAlexW3093929951

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.