ArticleMycoses2021
Identification of Candida auris and related species by multiplex PCR based on unique GPI protein-encoding genes.
Article in Mycoses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Diagnostic Approaches forMicroorganisms · 2025Review
- Current Updates on Molecular Diagnostic Assays Used for Detection ofDiagnostics (Basel, Switzerland) · 2025Review
- Review
- TheHealth science reports · 2024Article
- First Report of the Emerging PathogenJournal of fungi (Basel, Switzerland) · 2024Article
- The good, the bad, and the hazardous: comparative genomic analysis unveils cell wall features in the pathogen Candidozyma auris typical for both baker's yeast and Candida.FEMS yeast research · 2024Article
- A lateral flow immunoassay for the rapid identification ofFrontiers in microbiology · 2024Article
- Tools and techniques to identify, study, and control Candida auris.PLoS pathogens · 2023Review
- Raman Metabolomics ofInternational journal of molecular sciences · 2022Article
- The Menace ofDiseases (Basel, Switzerland) · 2022Review
- Tools for Detecting a "Superbug": Updates on Candida auris Testing.Journal of clinical microbiology · 2022Review
- ClaID: a Rapid Method of Clade-Level Identification of the Multidrug Resistant Human Fungal Pathogen Candida auris.Microbiology spectrum · 2022Article
- What Do We Know aboutMicroorganisms · 2021Review
- What happened to microbiological diagnosis in 2020 beyond COVID-19?Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2021Review
- Review
- So Many Diagnostic Tests, So Little Time: Review and Preview ofFrontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundThe pathogen Candida auris is rapidly gaining clinical importance because of its resistance to antifungal treatments and its persistence in hospital environments. Early and accurate diagnosis of C. auris infections is crucial, and however, the fungus has often been misidentified by commercial systems.
objectivesTo develop conventional and real-time PCR methods for accurate and rapid identification of C. auris and its discrimination from closely related species by exploiting the uniqueness of certain glycosylphosphatidylinositol (GPI)-modified protein-encoding genes.
methodsSpecies-specific primers for two unique putative GPI protein-encoding genes per species were designed for C. auris, C. haemulonii, C. pseudohaemulonii, C. duobushaemulonii, C. lusitaniae and C. albicans. Primers were blind tested for their specificity and efficiency in conventional and real-time multiplex PCR set-up.
resultsAll primers combinations showed excellent species specificity. In multiplex mode, correct identification was aided by different-sized amplicons for each species. Efficiency of the C. auris primers was validated using a panel of 155 C. auris isolates, including all known genetically diverse clades. In real-time multiplex PCR, different melting points of the amplicons allowed the distinction of C. auris from four related species. C. auris limit of detection was 5 CFU/reaction with a threshold value of 32. The method was also able to detect C. auris in spiked blood and serum.
conclusionsPCR identification based on unique GPI protein-encoding genes allows for accurate and rapid species identification of C. auris and related species without need for expensive equipment when applied in conventional PCR set-up.
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