ArticleNPJ biofilms and microbiomes2020
An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in Pseudomonas aeruginosa MPAO1.
Article in NPJ biofilms and microbiomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Article
- AMR-GNN: a multi-representation graph neural network framework to enable genomic antimicrobial resistance prediction.Nature communications · 2026Article
- UnderstandingMicroorganisms · 2026Review
- Microfluidics to Meet Antibiotic Resistance: A Growing Research Frontier.Antibiotics (Basel, Switzerland) · 2025Review
- Review
- High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance.Environmental microbiology · 2025Article
- Antibiotic-ResistantMicroorganisms · 2025Review
- A role for the stringent response in ciprofloxacin resistance in Pseudomonas aeruginosa.Scientific reports · 2024Article
- Article
- A Novel Robust Screening Assay IdentifiesMicroorganisms · 2023Article
- Genomic Analysis of the Rare Slightly Halophilic Myxobacterium "Microorganisms · 2023Article
- Boolean model of the gene regulatory network ofFrontiers in microbiology · 2023Article
- Unraveling the small proteome of the plant symbiontmicroLife · 2023Article
- In vitro Activity of Cefepime/Avibactam Against Carbapenem ResistantInfection and drug resistance · 2023Article
- Elucidation of the Bovine Intramammary Bacteriome and Resistome from healthy cows of Swiss dairy farms in the Canton Tessin.Frontiers in microbiology · 2023Article
- Inactivation of multiple human pathogens by Fathhome's dry sanitizer device: Rapid and eco-friendly ozone-based disinfection.Medicine in microecology · 2022Article
- Hybrid assembly using long reads resolves repeats and completes the genome sequence of a laboratory strain ofHeliyon · 2022Article
- Role of the flagellar hook in the structural development and antibiotic tolerance of Pseudomonas aeruginosa biofilms.The ISME journal · 2022Article
- Genome Mining and Analysis of PKS Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- A fluorescently labelled quaternary ammonium compound (NBD-DDA) to study resistance mechanisms in bacteria.Frontiers in microbiology · 2022Article
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14 authors.
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Abstract
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a genomics-driven workflow to assemble the complete MPAO1 genome, identify unique and conserved genes by comparative genomics with the PAO1 reference strain and genes missed within existing assemblies by proteogenomics. Among over 200 unique MPAO1 genes, we identified six general essential genes that were overlooked when mapping public Tn-seq data sets against PAO1, including an antitoxin. Genomic data were integrated with phenotypic data from an experimental workflow using a user-friendly, soft lithography-based microfluidic flow chamber for biofilm growth and a screen with the Tn-mutant library in microtiter plates. The screen identified hitherto unknown genes involved in biofilm growth and antibiotic resistance. Experiments conducted with the flow chamber across three laboratories delivered reproducible data on P. aeruginosa biofilms and validated the function of both known genes and genes identified in the Tn-mutant screens. Differential protein abundance data from planktonic cells versus biofilm confirmed the upregulation of candidates known to affect biofilm formation, of structural and secreted proteins of type VI secretion systems, and provided proteogenomic evidence for some missed MPAO1 genes. This integrated, broadly applicable model promises to improve the mechanistic understanding of biofilm formation, antimicrobial tolerance, and resistance evolution in biofilms.
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