ArticleThe British journal of ophthalmology2022
Eye bank versus surgeon prepared DMEK tissues: influence on adhesion and re-bubbling rate.
Article in The British journal of ophthalmology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Epithelial Thickness Changes After Descemet Membrane Endothelial Keratoplasty (DMEK): An Observational Study.Journal of clinical medicine · 2026Article
- Unlocking the surgical potential of Descemet's membrane: a standardized decellularization protocol.Cell and tissue banking · 2026Article
- Anterior Chamber Inflammation and Descemet Membrane Endothelial Keratoplasty: An Anterior Segment-OCT-Based Analysis.Ophthalmology science · 2026Article
- Preoperative and perioperative factors that predict graft failure 1 year after Descemet membrane endothelial keratoplasty.PloS one · 2026Article
- Multivariate relationships between graft detachment after DMEK and twelve pre/perioperative factors.Scientific reports · 2025Article
- A historical view of the development of corneal transplantation: from penetrating keratoplasty to selective transplantation of the finest corneal layers.Wiener medizinische Wochenschrift (1946) · 2025Review
- Graft detachment rates in surgeon-cut and pre-cut tissue for DSAEK transported in different mediums.Acta ophthalmologica · 2025Article
- Clinical outcomes in imported endothelium-in preloaded vs. surgeon-loaded DMEKs in Asian eyes.Frontiers in medicine · 2025Article
- Comparison of rebubbling rate between preloaded endothelium-in and preloaded no-touch endothelium-out Descemet membrane endothelial keratoplasty transplantation.BMC ophthalmology · 2024Article
- Donor Characteristics in Graft Detachment after Posterior Lamellar Keratoplasty for Fuchs Endothelial Dystrophy and Bullous Keratopathy.Journal of clinical medicine · 2024Article
- Predicting Long-Term Endothelial Cell Loss after Preloaded Descemet Membrane Endothelial Keratoplasty in Fuchs' Endothelial Corneal Dystrophy: A Mathematical Model.Journal of clinical medicine · 2024Article
- Measuring the viability of crystalline lens epithelial cells by triple Hoechst-Ethidium-Calcein-AM staining.Molecular vision · 2024Article
- Preoperative surgeon evaluation of corneal endothelial status: the Viability Control of Human Endothelial Cells before Keratoplasty (V-CHECK) study protocol.BMJ open ophthalmology · 2023Article
- Deep Learning Using Preoperative AS-OCT Predicts Graft Detachment in DMEK.Translational vision science & technology · 2023Article
- Ability of routinely collected clinical factors to predict good visual results after primary Descemet membrane endothelial keratoplasty: a cohort study.BMC ophthalmology · 2022Article
- Role of AS-OCT in Managing Corneal Disorders.Diagnostics (Basel, Switzerland) · 2022Review
- Eye bank versus surgeon prepared Descemet stripping automated endothelial keratoplasty tissues: Influence on adhesion force in a pilot study.Indian journal of ophthalmology · 2022Article
- "Endothelium-Out" and "Endothelium-In" Descemet Membrane Endothelial Keratoplasty (DMEK) Graft Insertion Techniques: A Systematic Review With Meta-Analysis.Frontiers in medicine · 2022Article
- Review for special issue: Corneal lamellar surgery: Present outcomes and future perspectives.Taiwan journal of ophthalmologyReview
- Clinical outcomes of prestripped, prestained, and preloaded Descemet's membrane endothelial keratoplasty ("P3 DMEK").Taiwan journal of ophthalmologyArticle
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the difference in adhesion and rebubbling rate between eye bank and surgeon prepared Descemet membrane endothelial keratoplasty (DMEK) tissues.
methodsLaboratory and clinical retrospective comparative interventional case series. Research corneal tissues were obtained for laboratory investigation. The clinical study involved patients with endothelial dysfunction who underwent DMEK surgery and tamponade with air. Tissues were stripped using a standard DMEK stripping technique (SCUBA) and shipped as prestripped or loaded in a 2.2 intra-ocular lens cartridge with endothelium facing inwards (preloaded) before transporting from the eye bank to the surgeon. For surgeon prepared tissues, all the grafts were stripped in the theatre and transplanted or stripped in the laboratory and tested immediately. Adhesion force and elastic modulus were measured in the centre and mid-periphery in a laboratory ex vivo investigation using atomic force microscopy, while rebubbling rates were recorded in the clinical study.
resultsThere was no difference in endothelial cell viability between surgeon or eye bank prepared tissue. Surgeon-stripped DMEK grafts in the laboratory investigation showed significantly higher elastic modulus and adhesion force compared to prestripped and preloaded tissues (p<0.0001). In the clinical data, rebubbling rates of 48%, 40% and 15% were observed in preloaded, prestripped and surgeon-stripped DMEK grafts, respectively. Rebubbling rates were significantly associated with combined cataract surgery (p=0.009) and with time from harvesting the graft to the surgery (p=0.02).
conclusionsDecreased adhesion forces and elastic modulus in eye bank prepared tissues may contribute to increased rebubbling rates.
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