ArticleCurrent microbiology2021
A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan).
Article in Current microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 77 citations in OpenAlex.
- Molecular characterization of Rhipicephalus microplus and tick-borne pathogens in cattle in Nepal.Veterinary research communications · 2025Article
- In Silico Analysis of Two Hard Tick P450s: Identification, Characterization, and Putative Metabolism ofInternational journal of molecular sciences · 2025Article
- High Prevalence and Genetic Heterogeneity ofPathogens (Basel, Switzerland) · 2025Article
- A Cross-Sectional Serological Study to Assess the Prevalence and Risk Factors of Anaplasmosis in Dromedary Camels in Punjab, Pakistan.Veterinary sciences · 2024Article
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Tick-borneFrontiers in microbiology · 2024Article
- Molecular surveillance and genetic diversity of Anaplasma spp. in cattle (Bos taurus) and goat (Capra aegagrus hircus) from Hainan island/province, China.BMC veterinary research · 2023Article
- Molecular Epidemiology, Seasonality and Phylogenetic Investigations ofMicroorganisms · 2023Article
- Hematobiochemical, serological, and molecular detection ofVeterinary world · 2023Article
- Molecular detection and characterization ofFrontiers in veterinary science · 2023Article
- Article
- Seasonal Investigation ofPathogens (Basel, Switzerland) · 2022Article
- Prevalence ofSaudi journal of biological sciences · 2022Article
- Prevalence, epidemiology, seasonality, and phylogeny of Anaplasma marginale in blood samples of goats collected from Punjab, Pakistan.Tropical animal health and production · 2022Article
- Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan.Tropical animal health and production · 2021Article
- Molecular detection and phylogenetic analyses of Anaplasma spp. in Haemaphysalis longicornis from goats in four provinces of China.Scientific reports · 2021Article
- An Epidemiological Survey Regarding Ticks and Tick-Borne Diseases among Livestock Owners in Punjab, Pakistan: A One Health Context.Pathogens (Basel, Switzerland) · 2021Article
- Molecular epidemiology of Theileria annulata infection of cattle in Layyah District, Pakistan.Experimental & applied acarology · 2021Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
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Abstract
Anaplasmosis is a tick-borne disease caused by obligate intercellular gram-negative bacteria, Anaplasma (A.) marginale. The present study reports on seasonal prevalence, epidemiology, and phylogeny of A. marginale in three cattle breeds from District Layyah, Southern Punjab, Pakistan. A total of 844 blood samples (Cross = 300, Holstein Friesian = 244, Sahiwal breed = 300) from apparently healthy cattle on seasonal basis were collected along with epidemiological data during May 2018 till April 2019. Polymerase chain reaction generated 265 base-pair amplicon specific for major surface protein-1b encoding gene of A. marginale in 8.6% (73/844) of enrolled cattle. The highest prevalence was observed during autumn (18.3%) followed by summer (9.7%) and winter season (7.1%). Holstein Friesian breed was most susceptible to A. marginale infection (13.1%) followed by Sahiwal (7.6%) and cross breed (6%). Representative amplified partial gene sequences of A. marginale were submitted to GenBank (Accession numbers MK032842 and MK032843). 37/844 (4.3%) Giemsa-stained blood smears were found positive for Anaplasma spp. Small number of ticks including Hyalomma anatolicum, Hyalomma excavatum, Rhipicephalus microplus, Haemaphysalis punctata were identified from cattle but none of them was found PCR positive for the presence of A. marginale. Analysis of epidemiological factors revealed that female cattle and farm with water supply from pool, farms where other dairy animals and dogs were living with cattle and dogs having ticks load on them had significant association with A. marginale prevalence. It was observed that white blood cell, lymphocytes (%), monocytes (%) hematocrit, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration were significantly disturbed in A. marginale-positive than negative cattle.
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