ArticleInternational journal of molecular medicine2020
PTH(1‑34) activates the migration and adhesion of BMSCs through the rictor/mTORC2 pathway.
Article in International journal of molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Inflammation and Dysregulated Bone Turnover Confound Serum ICAM-1 as a Cardiovascular Marker in Hemodialysis.Biomolecules · 2026Article
- G protein-coupled receptor signaling in osteogenic bone mesenchymal stem/stromal cells.Frontiers in cell and developmental biology · 2026Review
- Local abaloparatide administration promotes in situ alveolar bone augmentation via FAK-mediated periosteal osteogenesis.International journal of oral science · 2025Article
- PTH Promotes Chondrogenesis of Fibrocartilage Stem Cells and Alleviates Temporomandibular Joint Osteoarthritis.Tissue engineering and regenerative medicine · 2025Article
- Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling.eLife · 2024Article
- Review
- Human Parathyroid Hormone Analog (3-34/29-34) promotes wound re-epithelialization through inducing keratinocyte migration and epithelial-mesenchymal transition via PTHR1-PI3K/AKT activation.Cell communication and signaling : CCS · 2023Article
- Graphene Oxide/RhPTH(1-34)/Polylactide Composite Nanofibrous Scaffold for Bone Tissue Engineering.International journal of molecular sciences · 2023Article
- Hormone and implant osseointegration: Elaboration of the relationship among function, preclinical, and clinical practice.Frontiers in molecular biosciences · 2022Review
- Parathyroid Hormone 1 Receptor Signaling in Dental Mesenchymal Stem Cells: Basic and Clinical Implications.Frontiers in cell and developmental biology · 2021Review
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of intermittent parathyroid hormone (1‑34) [PTH(1‑34)] treatment to enhance bone‑implant osseointegration was recently demonstrated in vivo. However, the mechanisms through which PTH (1‑34) regulates bone marrow‑derived stromal cells (BMSCs) remain unclear. The present study thus aimed to investigate the effects of PTH(1‑34) on the migration and adhesion of, and rictor/mammalian target of rapamycin complex 2 (mTORC2) signaling in BMSCs. In the present study, BMSCs were isolated from Sprague‑Dawley rats treated with various concentrations of PTH(1‑34) for different periods of time. PTH(1‑34) treatment was performed with or without an mTORC1 inhibitor (20 nM rapamycin) and mTORC1/2 inhibitor (10 µM PP242). Cell migration was assessed by Transwell cell migration assays and wound healing assays. Cell adhesion and related mRNA expression were investigated through adhesion assays and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), respectively. The protein expression of chemokine receptors (CXCR4 and CCR2) and adhesion factors [intercellular adhesion molecule 1 (ICAM‑1), fibronectin and integrin β1] was examined by western blot analysis. The results revealed that various concentrations (1, 10, 20, 50 and 100 nM) of PTH(1‑34) significantly increased the migration and adhesion of BMSCs, as well as the expression of CXCR4, CCR2, ICAM‑1, fibronectin and integrin β1. In addition, the p‑Akt and p‑S6 levels were also upregulated by PTH(1‑34). BMSCs subjected to mTORC1/2 signaling pathway inhibition or rictor silencing exhibited a markedly reduced PTH‑induced migration and adhesion, while no such effect was observed for the BMSCs subjected to mTORC1 pathway inhibition or raptor silencing. These results indicate that PTH(1‑34) promotes BMSC migration and adhesion through rictor/mTORC2 signaling in vitro. Taken together, the results of the present study reveal an important mechanism for the therapeutic effects of PTH(1‑34) on bone‑implant osseointegration and suggest a potential treatment strategy based on the effect of PTH(1‑34) on BMSCs.
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