Evidence map›Paper›PMID 33125091›Full record

Observational studyOncology reports2020

Sevoflurane suppresses cell viability and invasion and promotes cell apoptosis in colon cancer by modulating exosome‑mediated circ‑HMGCS1 via the miR‑34a‑5p/SGPP1 axis.

Juan He, Huaping Zhao, Xing Liu, Dongmei Wang, Yong Wang, Yanqiu Ai, Jianjun Yang

Abstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Oncology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Article
  2. Review
  3. Sevoflurane induces CaCytotechnology · 2025
    Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. microRNA-92b-3p augments colon cancer development through inhibiting KLF3.Journal of biochemical and molecular toxicology · 2023
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Juan HeDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
Huaping ZhaoDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
Xing LiuDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
Dongmei WangDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
Yong WangDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
Yanqiu AiDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
Jianjun YangDepartment of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As a novel halogenated hydroxyl ether‑inhaled general anesthetic, sevoflurane has been reported to affect the progression of diverse human cancers. In the present study, we aimed to explore the functions and underlying mechanisms of sevoflurane in colon cancer. MTT assay, flow cytometric analysis and Transwell assay were conducted to evaluate cell viability, apoptosis and invasion, respectively. Western blot analysis was performed to determine the protein level of sphingosine‑1‑phosphate phosphatase 1 (SGPP1). The morphology and size of exosomes were analyzed by TEM and NTA. The levels of circular RNA 3‑hydroxy‑3‑methylglutaryl‑CoA synthase 1 (circ‑HMGCS1), microRNA (miR)‑34a‑5p and SGPP1 mRNA were examined by RT‑qPCR. Dual‑luciferase reporter and RNA RIP assays were utilized to explore the interaction between miR‑34a‑5p and circ‑HMGCS1 or SGPP1. A murine xenograft model was established to investigate the effect of circ‑HMGCS1 in vivo. As a result, it was determined that sevoflurane suppressed cell viability and invasion and induced apoptosis in colon cancer in a dose‑dependent way. Exosomal circ‑HMGCS1 was increased in the serums and cells of colon cancer patients. Circ‑HMGCS1 was downregulated by sevoflurane treatment in colon cancer cells and circ‑HMGCS1 overexpression could restore the effect of sevoflurane on colon cancer cell development. miR‑34a‑5p was a target of circ‑HMGCS1 and miR‑34a‑5p inhibition reversed the effect of circ‑HMGCS1 silencing on colon cancer cell progression. Moreover, circ‑HMGCS1 knockdown suppressed SGPP1 expression via sponging miR‑34a‑5p. Knockdown of circ‑HMGCS1 blocked tumor growth in vivo. In conclusion, sevoflurane inhibited colon cancer progression by modulating the exosome‑transmitted circ‑HMGCS1/miR‑34a‑5p/SGPP1 axis.

Indexed as

AgedAnimalsApoptosisCase-Control StudiesCell Line, TumorCell MovementCell SurvivalColonColonic NeoplasmsDisease ProgressionExosomesFemaleGene Expression Regulation, NeoplasticGene Knockdown TechniquesHealthy VolunteersHumansMembrane ProteinsMicroRNAsMIRN34 microRNA, humanPhosphoric Monoester HydrolasesRNA, CircularSevofluraneSGPP1 protein, humancirc‑HMGCS1colon cancerexosomemiR‑34a‑5psevofluraneSGPP1

Identifiers

PMID33125091
PMCPMC7610314

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.