ArticleThe Plant journal : for cell and molecular biology2021
Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves.
Article in The Plant journal : for cell and molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana.The New phytologist · 2026Article
- OsRALF26 Serves as an Endogenous Signal Recognised by XA21 to Promote Robust and Distal Resistance in Rice.Plant biotechnology journal · 2026Article
- A disease resistance assay in Nicotiana benthamiana reveals the immune function of Response to HopBA1.Plant physiology · 2024Article
- Multifaceted roles of plant glycosyl hydrolases during pathogen infections: more to discover.Planta · 2024Review
- Activity-based proteomics uncovers suppressed hydrolases and a neo-functionalised antibacterial enzyme at the plant-pathogen interface.The New phytologist · 2024Article
- Mapping yield and yield-related traits using diverse common bean germplasm.Frontiers in genetics · 2023Article
- A conserved glutamate residue in RPM1-INTERACTING PROTEIN4 is ADP-ribosylated by the Pseudomonas effector AvrRpm2 to activate RPM1-mediated plant resistance.The Plant cell · 2022Article
- Loss of function of the bHLH transcription factor Nrd1 in tomato enhances resistance to Pseudomonas syringae.Plant physiology · 2022Article
- Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme.The New phytologist · 2022Article
- Monitoring Pseudomonas syringae Growth in Agroinfiltrated Leaves: The "Agromonas" Assay.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The lengthy process to generate transformed plants is a limitation in current research on the interactions of the model plant pathogen Pseudomonas syringae with plant hosts. Here we present an easy method called agromonas, where we quantify P. syringae growth in agroinfiltrated leaves of Nicotiana benthamiana using a cocktail of antibiotics to select P. syringae on plates. As a proof of concept, we demonstrate that transient expression of PAMP receptors reduces bacterial growth, and that transient depletion of a host immune gene and transient expression of a type-III effector increase P. syringae growth in agromonas assays. We show that we can rapidly achieve structure-function analysis of immune components and test the function of immune hydrolases. The agromonas method is easy, fast and robust for routine disease assays with various Pseudomonas strains without transforming plants or bacteria. The agromonas assay offers a reliable approach for further comprehensive analysis of plant immunity.
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Registered trials
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