Evidence map›Paper›PMID 33123939›Full record

ArticleDigestive diseases and sciences2021

Protective Mechanism of MIF Inhibitor ISO-1 on Intrahepatic Bile Duct Cells in Rats with Severe Acute Pancreatitis.

Bin Wang, Kailiang Zhao, Wenjuan Hu, Youming Ding, Weixing Wang

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Article in Digestive diseases and sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Bin WangHubei Key Laboratory of Digestive System Disease, Department of Hepatobiliary Surgery, Renminbi Hospital of Wuhan University, Wuhan, 430060, Hubei Province, China. wb7112@126.com.ORCID 0000-0001-6450-8792
Kailiang ZhaoHubei Key Laboratory of Digestive System Disease, Department of Hepatobiliary Surgery, Renminbi Hospital of Wuhan University, Wuhan, 430060, Hubei Province, China.
Wenjuan HuDepartment of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, China.
Youming DingHubei Key Laboratory of Digestive System Disease, Department of Hepatobiliary Surgery, Renminbi Hospital of Wuhan University, Wuhan, 430060, Hubei Province, China.
Weixing WangHubei Key Laboratory of Digestive System Disease, Department of Hepatobiliary Surgery, Renminbi Hospital of Wuhan University, Wuhan, 430060, Hubei Province, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsThis study aimed to explore the protection mechanism of ISO-1 on severe acute pancreatitis-associated intrahepatic bile duct (IBD) injury in rats.

methodsForty-eight specific-pathogen-free male Wistar rats were randomly divided into four groups (N = 12): a sham operation group (SO group), a severe acute pancreatitis model group (SAP group), a ISO-1 treatment group (ISO-1 + SAP group), and a ISO-1 control group (ISO-1 + SO group). All rats were killed after 12 h of being made models. Immunohistochemistry was used to detect the expression of MIF and P38 in IBD cells. MIF mRNA expression in IBD cells was observed using real-time fluorescent quantitative polymerase chain reaction (real-time PCR). In addition, Western blotting was performed to detect the protein expression of P38, phosphorylated P38 (P-P38), nuclear factor-κB (NF-κB p65), and tumor necrosis factor alpha (TNF-α). Enzyme-linked immunosorbent assays were used to analyze the levels of TNF-α, IL-1β, and IL-6 in the IBD of rats.

resultsCompared with SAP, after treatment with ISO-1, the pathological injuries of pancreas, liver, and IBD cells in ISO-1 treatment group remarkably relieved. The expression of MIF in the IBD cells was significantly downregulated both at mRNA and at protein levels in ISO-1 treatment group. Besides, the protein expression levels of P38, P-P38, NF-κBp65, TNF-α, IL-1β, and IL-6 in the IBD in rats were also significantly decreased in ISO-1 treatment group (all P < 0.05).

conclusionISO-1 may protect the IBD cells, reduce pathological injuries, and reduce the inflammatory response in SAP rats. Its mechanisms may be via inhibiting the expression of MIF and then blocking the activation of p38-MAPK and NF-κB signaling pathway.

Indexed as

Acute DiseaseAnimalsBile Ducts, IntrahepaticCytokinesGene Expression RegulationIntramolecular OxidoreductasesIsoxazolesMacrophage Migration-Inhibitory FactorsMalePancreatitisRandom AllocationRatsRats, WistarReal-Time Polymerase Chain ReactionSpecific Pathogen-Free Organisms3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazoleacetic acid methyl esterCytokinesIntramolecular OxidoreductasesIsoxazolesMacrophage Migration-Inhibitory FactorsMif protein, ratCytoprotectionIntrahepatic bile duct cellMacrophage migration inhibitory factor inhibitorP38Severe acute pancreatitis

Identifiers

PMID33123939

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.