Diet-induced obesity attenuates the hypothermic response to lipopolysaccharide independently of TNF-α production.

Life-threatening infections (sepsis) are usually associated with co-morbidities, among which obesity deserves attention. Here, we evaluated whether and how obesity affects the switch from fever to hypothermia that occurs in the most severe cases of sepsis, which is thought to provide physiological support for a change in host defense strategy from resistance to tolerance. Obesity was induced by keeping rats on a high-fat diet for 32-34 weeks. The hypothermia induced by a high dose of bacterial lipopolysaccharide (LPS, 300 μg/animal, i.a.) was attenuated in the obese rats, as compared to their low-fat diet counterparts. Surprisingly, such attenuation occurred in spite of an enhancement in the circulating level of TNF-α, the most renowned mediator of LPS-induced hypothermia. Hence, it seems that factors counteracting not the production, but rather the action of TNF-α are at play in rats with diet-induced obesity. One of these factors might be IL-1β, a febrigenic mediator that also had its circulating levels augmented in the obese rats challenged with LPS. Taken together with previous reports of diet-induced obesity enhancing the fever induced by lower doses of LPS, the results of the present study indicate that obesity biases host defense toward a fever/resistance strategy,