ReviewFrontiers in neuroscience2020
Tissue Clearing and Expansion Methods for Imaging Brain Pathology in Neurodegeneration: From Circuits to Synapses and Beyond.
Review in Frontiers in neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- A Novel Rapid 3D Tissue-Clearing and Staining Approach for Enteric Neurovascular Imaging and Pathology Applications.Diagnostics (Basel, Switzerland) · 2026Article
- A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging.Experimental & molecular medicine · 2025Article
- Cutting-edge Strategies for Overcoming Therapeutic Barriers in Alzheimer's Disease.Current pharmaceutical design · 2025Review
- Article
- Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue.PNAS nexus · 2024Article
- iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-Throughput Imaging in a Mouse Model of Traumatic Brain Injury.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy.The Journal of cell biology · 2023Review
- Expansion microscopy: A chemical approach for super-resolution microscopy.Current opinion in structural biology · 2023Review
- Recent advances in understanding neuronal diversity and neural circuit complexity across different brain regions using single-cell sequencing.Frontiers in neural circuits · 2023Review
- Tissue clearing applications in memory engram research.Frontiers in behavioral neuroscience · 2023Review
- Review
- Spatiotemporal characterization of cellular tau pathology in the human locus coeruleus-pericoerulear complex by three-dimensional imaging.Acta neuropathologica · 2022Article
- Obesity-Related Neuroinflammation: Magnetic Resonance and Microscopy Imaging of the Brain.International journal of molecular sciences · 2022Review
- Challenges and advances in optical 3D mesoscale imaging.Journal of microscopy · 2022Article
- Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging.Photoacoustics · 2022Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- Imaging Synaptic Density: The Next Holy Grail of Neuroscience?Frontiers in neuroscience · 2022Review
- Towards organism-level systems biology by next-generation genetics and whole-organ cell profiling.Biophysical reviews · 2021Review
- Towards a Comprehensive Optical Connectome at Single Synapse ResolutionFrontiers in synaptic neuroscience · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying the structural alterations occurring during diseases of the nervous system requires imaging heterogeneous cell populations at the circuit, cellular and subcellular levels. Recent advancements in brain tissue clearing and expansion methods allow unprecedented detailed imaging of the nervous system through its entire scale, from circuits to synapses, including neurovascular and brain lymphatics elements. Here, we review the state-of-the-art of brain tissue clearing and expansion methods, mentioning their main advantages and limitations, and suggest their parallel implementation for circuits-to-synapses brain imaging using conventional (diffraction-limited) light microscopy -such as confocal, two-photon and light-sheet microscopy- to interrogate the cellular and molecular basis of neurodegenerative diseases. We discuss recent studies in which clearing and expansion methods have been successfully applied to study neuropathological processes in mouse models and postmortem human brain tissue. Volumetric imaging of cleared intact mouse brains and large human brain samples has allowed unbiased assessment of neuropathological hallmarks. In contrast, nanoscale imaging of expanded cells and brain tissue has been used to study the effect of protein aggregates on specific subcellular structures. Therefore, these approaches can be readily applied to study a wide range of brain processes and pathological mechanisms with cellular and subcellular resolution in a time- and cost-efficient manner. We consider that a broader implementation of these technologies is necessary to reveal the full landscape of cellular and molecular mechanisms underlying neurodegenerative diseases.
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