ArticleScientific reports2020
Improvements in the production of purified M13 bacteriophage bio-nanoparticle.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Functional Blood-Brain Barrier Crossing by Biomimetic M13 Phage Vectors for Targeted Neuronal Delivery.Advanced healthcare materials · 2026Article
- Mixed Ionic and Electronic Charge Transport in Conductive Protein Fibers Revealed with DC Electrical Measurements.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- A Synthetic Phage-Peptide Conjugate as a Potent Antibacterial Agent forACS central science · 2025Article
- Metagenome-inspired libraries to engineer phage M13 for targeted killing of Gram-negative bacterial species.Nucleic acids research · 2025Article
- Phage-Templated Synthesis of Targeted Photoactive 1D-Thiophene Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Diversification of Phage-Displayed Peptide Libraries with Noncanonical Amino Acid Mutagenesis and Chemical Modification.Chemical reviews · 2024Review
- Bacterial isolation and genome analysis of a novel Klebsiella quasipneumoniae phage in southwest China's karst area.Virology journal · 2024Article
- A modular phage vector platform for targeted photodynamic therapy of Gram-negative bacterial pathogens.iScience · 2023Article
- Optimisation of GraPhage13 macro-dispersibilityNanoscale · 2023Article
- Determination of the Dissociation Constant for Polyvalent Receptors Using ELISA: A Case of M13 Phages Displaying Troponin T-Specific Peptides.ACS omega · 2023Article
- Structurally Modified Plant Viruses and Bacteriophages with Helical Structure. Properties and Applications.Biochemistry. Biokhimiia · 2022Review
- Graphene Oxide and Biomolecules for the Production of Functional 3D Graphene-Based Materials.Frontiers in molecular biosciences · 2022Review
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Authors and funding
4 authors.
Funding
Abstract
M13 bacteriophage is a well-established versatile nano-building block, which can be employed to produce novel self-assembled functional materials and devices. Sufficient production and scalability of the M13, often require a large quantity of the virus and thus, improved propagation methods characterised by high capacity and degree of purity are essential. Currently, the 'gold-standard' is represented by infecting Escherichia coli cultures, followed by precipitation with polyethylene glycol (PEG). However, this is considerably flawed by the accumulation of contaminant PEG inside the freshly produced stocks, potentially hampering the reactivity of the individual M13 filaments. Our study demonstrates the effectiveness of implementing an isoelectric precipitation procedure to reduce the residual PEG along with FT-IR spectroscopy as a rapid, convenient and effective analytic validation method to detect the presence of this contaminant in freshly prepared M13 stocks.
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