ArticleThe Journal of cell biology1987
Inhibition of nucleolar reformation after microinjection of antibodies to RNA polymerase I into mitotic cells.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 109 citations in OpenAlex.
- ArcRNAs and the formation of nuclear bodies.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function.PloS one · 2014Article
- Biogenesis of nuclear bodies.Cold Spring Harbor perspectives in biology · 2010Review
- Nucleolar separation from chromosomes during Aspergillus nidulans mitosis can occur without spindle forces.Molecular biology of the cell · 2009Article
- Positioning of NORs and NOR-bearing chromosomes in relation to nucleoli.Journal of structural biology · 2007Article
- Nucleolar adaptation in human cancer.Cancer investigation · 2005Review
- Human acrocentric chromosomes with transcriptionally silent nucleolar organizer regions associate with nucleoli.The EMBO journal · 2001Article
- Nucleolar assembly of the rRNA processing machinery in living cells.The Journal of cell biology · 2001Article
- The dynamics of postmitotic reassembly of the nucleolus.The Journal of cell biology · 2000Article
- Initiation of nucleolar assembly is independent of RNA polymerase I transcription.Molecular biology of the cell · 2000Article
- Assembly and functional organization of the nucleolus: ultrastructural analysis of Saccharomyces cerevisiae mutants.Molecular biology of the cell · 2000Article
- The ribosomal RNA processing machinery is recruited to the nucleolar domain before RNA polymerase I during Xenopus laevis development.The Journal of cell biology · 2000Article
- Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis.The Journal of cell biology · 1998Article
- The role of the Schizosaccharomyces pombe gar2 protein in nucleolar structure and function depends on the concerted action of its highly charged N terminus and its RNA-binding domains.Molecular biology of the cell · 1998Article
- M phase phosphoprotein 10 is a human U3 small nucleolar ribonucleoprotein component.Molecular biology of the cell · 1998Article
- Article
- Review
- Nucleolar organizer expression in Allium cepa L. chromosomes.Chromosoma · 1996Article
- The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs.The Journal of cell biology · 1996Article
- Post-mitotic assembly of the nucleolus. I. The internal matrix network is a recruitment site for processing nucleolar components in prenucleolar bodies.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of daughter nuclei and the reformation of nucleolar structures was studied after microinjection of antibodies to RNA polymerase I into dividing cultured cells (PtK2). The fate of several nucleolar proteins representing the three main structural subcomponents of the nucleolus was examined by immunofluorescence and electron microscopy. The results show that the RNA polymerase I antibodies do not interfere with normal mitotic progression or the early steps of nucleologenesis, i.e., the aggregation of nucleolar material into prenucleolar bodies. However, they inhibit the telophasic coalescence of the prenucleolar bodies into the chromosomal nucleolar organizer regions, thus preventing the formation of new nucleoli. These prenucleolar bodies show a fibrillar organization that also compositionally resembles the dense fibrillar component of interphase nucleoli. We conclude that during normal nucleologenesis the dense fibrillar component forms from preformed entities around nucleolar organizer regions, and that this association seems to be dependent on the presence of an active form of RNA polymerase I.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.