ArticleInfection and immunity1987
Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation.
Article in Infection and immunity, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 138 papers.
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Who cites it
138 citing papers in PubMed, 329 citations in OpenAlex.
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- CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing.mSystems · 2023Article
- Cellular stress promotes NOD1/2-dependent inflammation via the endogenous metabolite sphingosine-1-phosphate.The EMBO journal · 2021Article
- Physical Constraints and Forces Involved in Phagocytosis.Frontiers in immunology · 2020Review
- XIAP controls RIPK2 signaling by preventing its deposition in speck-like structures.Life science alliance · 2019Article
- Synthetic bottom-up approach reveals the complex interplay ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Evolution of polymer formation within the actin superfamily.Molecular biology of the cell · 2017Review
- Injection of T3SS effectors not resulting in invasion is the main targeting mechanism ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mechanisms of Horizontal Cell-to-Cell Transfer of Wolbachia spp. in Drosophila melanogaster.Applied and environmental microbiology · 2017Article
- Analysis of host microRNA function uncovers a role for miR-29b-2-5p in Shigella capture by filopodia.PLoS pathogens · 2017Article
- Genetic Dissection of the Signaling Cascade that Controls Activation of the Shigella Type III Secretion System from the Needle Tip.Scientific reports · 2016Article
- The type III secretion system apparatus determines the intracellular niche of bacterial pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- How Shigella Utilizes Ca(2+) Jagged Edge Signals during Invasion of Epithelial Cells.Frontiers in cellular and infection microbiology · 2016Review
78 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enteroinvasive bacterium Shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. By using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (NBD-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of HeLa cells which interacted with the invasive isolate M90T. As demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody CC-212, myosin accumulated at the same sites. The entry process could be synchronized by using strain SC301, a pIL22 transformant of M90T. pIL22, a recombinant plasmid encoding the Escherichia coli afimbrial adhesin AFA I, rendered shigellae highly adherent to HeLa cells. Using such a system, we demonstrated that the occurrence of bacterial penetration and the appearance of structures brightly stained by NBD-phallacidin were simultaneous events. Such microfilamentous structures resulted from de novo polymerization of the monomeric actin pool in a DNase I inhibition assay, as shown by measurement of the monomeric versus total actin content of infected HeLa cells. These data provide direct evidence that the penetration of S. flexneri into HeLa cells occurs through a mechanism similar to phagocytosis by professional phagocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.